Eicosanoid regulation of vascular endothelial growth factor expression and angiogenesis in microvessel endothelial cells.

The Journal of Biological Chemistry
Alexander MezentsevMichal Laniado-Schwartzman

Abstract

12(R)-Hydroxy-5,8,14-eicosatrienoic acid (HETrE) is a potent inflammatory and angiogenic eicosanoid in ocular and dermal tissues. Previous studies suggested that 12(R)-HETrE activates microvessel endothelial cells via a high affinity binding site; however, the cellular mechanisms underlying 12(R)-HETrE angiogenic activity are unexplored. Because the synthesis of 12(R)-HETrE is induced in response to hypoxic injury, we examined its interactions with vascular endothelial growth factor (VEGF) in rabbit limbal microvessel endothelial cells. Addition of 12(R)-HETrE (0.1 nm) to the cells increased VEGF mRNA levels with maximum 5-fold increase at 45 min. The increase in VEGF mRNA was followed by an increase in immunoreactive VEGF protein. 12(R)-HETrE (0.1 nm) rapidly activated the extracellular signal-regulated kinases (ERKs) ERK1 and ERK2. Moreover, preincubation of cells with PD98059, a selective inhibitor of MEK-1, inhibited 12(R)-HETrE-induced VEGF mRNA. Addition of VEGF antibody to cells grown in Matrigel-coated culture plates inhibited 12(R)-HETrE-induced capillary tube-like formation, suggesting that VEGF mediates, at least in part, the angiogenic response to 12(R)-HETrE. The results indicate that in microvessel endothelial cel...Continue Reading

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Citations

Sep 24, 2009·The Journal of Pharmacology and Experimental Therapeutics·Lars BellnerMichal Laniado Schwartzman
Jan 8, 2013·Prostaglandins & Other Lipid Mediators·Yossi IssanMichal Laniado-Schwartzman
Nov 28, 2008·Bioorganic & Medicinal Chemistry·Takahiro YamashitaNobuhiro Fusetani
Nov 10, 2007·Prostaglandins & Other Lipid Mediators·Francesca SetaMichal Laniado Schwartzman
Sep 19, 2003·Biochemical and Biophysical Research Communications·Indira NeeliGadiparthi N Rao
Oct 31, 2006·The American Journal of Pathology·Francesca SetaMichal Laniado-Schwartzman
Nov 19, 2011·Blood·Lalitha Nayak, Mukesh K Jain
Dec 13, 2003·Circulation Research·Miao JiangMichal Laniado-Schwartzman
Nov 19, 2004·The Journal of Biological Chemistry·Nagadhara DronadulaGadiparthi N Rao
Jul 13, 2005·The Journal of Pharmacology and Experimental Therapeutics·Alexandre MezentsevMichal Laniado-Schwartzman
Nov 12, 2015·World Journal of Gastroenterology : WJG·Sinem Tuncer, Sreeparna Banerjee
Jul 28, 2020·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Youjia DuPeter Zahradka
Nov 26, 2018·Progress in Lipid Research·Nikhlesh K Singh, Gadiparthi N Rao
Mar 9, 2010·European Journal of Cell Biology·Raul Martinez-OrozcoEduardo Perez Salazar

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