Eicosanoid synthesis in human peritoneal macrophages stimulated with S. epidermidis

Kidney International
R MackenzieJ D Williams

Abstract

Peritoneal macrophages isolated from CAPD patients phagocytosed S. epidermidis in a time dependent manner. Coincident with a maximum phagocytic uptake of 56% by 12 hours, there was secretion of a significant amount of neutral protease (1.37 +/- 0.2 mg [3H]-casein degraded/10(6) cells, P = 0.05). In contrast to these delayed effects, coincubation of PMO with S. epidermidis resulted in a significant increase in both LTB4 and LTC4 synthesis above that of controls, with 6.33 +/- 1.20 ng LTB4/10(6) cells (P less than 0.01) and 2.06 +/- 0.68 ng LTC4/10(6) cells (P = 0.014) being generated by three hours. The generation of these lipoxygenase products was both time and dose dependent, and the rapid production and release of the potently chemotactic LTB4 is consistent with the observed clinical response, where a rapid influx of PMN into the peritoneal cavity occurs during episodes of peritonitis, while the generation of LTC4 may contribute to the hyperemia and interstitial edema. In contrast, although there was a time dependent rise in cyclooxygenase product generation by unstimulated cells, a dose dependent inhibition of synthesis was clearly demonstrated when cells were incubated with bacteria, with a mean 40% reduction in generation ...Continue Reading

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Citations

Oct 1, 1995·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·J S Cameron
Oct 1, 1996·Immunobiology·N TopleyJ D Williams
Jan 1, 1997·The Journal of Laboratory and Clinical Medicine·R K MackenzieJ D Williams
Feb 4, 2009·Laboratory Investigation; a Journal of Technical Methods and Pathology·Janusz WitowskiAchim Jörres
Oct 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Suzanne M HurstSimon A Jones
Apr 23, 2003·Journal of the American Society of Nephrology : JASN·Siska MortierNorbert H Lameire
Jan 6, 2000·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·R K MackenzieN Topley

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