PMID: 8605728Mar 1, 1996Paper

Eicosanoids as mediators of linoleic acid-stimulated invasion and type IV collagenase production by a metastatic human breast cancer cell line

Clinical & Experimental Metastasis
X H LiuD P Rose

Abstract

Diets rich in linoleic acid (LA) stimulate the metastasis of MDA-MB-435 human breast cancer cells from the mammary fat pads of nude mice. This omega-6 fatty acid is metabolized to various cyclo-oxygenase and lipoxygenase products, several of which have been previously associated with tumor cell invasion and metastasis. We now report that MDA-MB-435 cells secreted increased levels of prostaglandin E2 (PGE2), and 12-hydroxyeicosatetraenoic acid (12-HETE) and 15-HETE when cultured in the presence of 2.7 microM (0.75 micrograms/ml) LA; 5-HETE secretion was unchanged. The 12-lipoxygenase inhibitor esculetin (20 microM) completely blocked the LA-stimulated 12-HETE secretion. Linoleic acid also increased MDA-MB-435 cell invasion in an in vitro assay; this stimulation was abolished by 20 microM esculetin, but was unaffected by piroxicam, a selective cyclooxygenase inhibitor. The effect of LA on invasion was replicated by 0.1 microM 12-HETE, but not by 5-HETE or PGE2; 15-HETE was stimulatory only at a concentration of 1.0 microM. Zymographic and Northern blot analyses showed that these events are accompanied by the induction of 92 kDa isoform type IV collagenase (metalloproteinase-9) enzymic activity and mRNA expression by exogenous LA ...Continue Reading

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Citations

Mar 25, 1997·Breast Cancer : the Journal of the Japanese Breast Cancer Society·D P Rose
Oct 31, 2013·Journal of Mammary Gland Biology and Neoplasia·Linda Vona-Davis, David P Rose
Nov 4, 2000·The Journal of Urology·V L FreemanE M Wojcik
Aug 9, 2003·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Wen G JiangRobert E Mansel
Jul 3, 1998·Critical Reviews in Oncology/hematology·W G JiangD F Horrobin
Mar 28, 1998·Breast Cancer Research and Treatment·D P Rose, J M Connolly
Mar 24, 2005·Bioscience, Biotechnology, and Biochemistry·Nobuhiro HirakawaKazumi Yagasaki
Nov 4, 2000·Expert Opinion on Investigational Drugs·V E SteeleG J Kelloff
Jun 14, 2005·Biochemical Pharmacology·Khosrow Kashfi, Basil Rigas
Jun 23, 2015·Surgical Neurology International·Russell L Blaylock
Sep 16, 2009·Laboratory Investigation; a Journal of Technical Methods and Pathology·Chang Hyun ByonYabing Chen
Aug 28, 2007·Applied Immunohistochemistry & Molecular Morphology : AIMM·Aziza NassarCynthia Cohen
Jan 6, 2001·Nutrition and Cancer·D P Rose, J M Connolly
Aug 23, 2000·The Journal of Biological Chemistry·C K SzekeresK V Honn
Mar 12, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kenneth V HonnStephanie C Tucker
Nov 13, 2018·World Journal of Gastroenterology : WJG·Yu-Ming RongPing Lan
Sep 19, 2019·Laboratory Animals·Wilko ThieleJonathan P Sleeman
Mar 25, 2019·Biochemical Society Transactions·Monica Napolitano

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