Eicosanoids produced during interactions between Pseudomonas aeruginosa and alveolar macrophages are species-dependent

Immunology and Cell Biology
T C SorrellM Müller

Abstract

Eicosanoid production during phagocytosis of pyogenic bacteria by rabbit alveolar macrophages was studied as a model of early events in the pathogenesis of pneumonia. Adherent alveolar macrophages, prelabelled with [3H]-arachidonic acid (AA), were incubated with live, opsonized Staphylococcus aureus or Pseudomonas aeruginosa (bacteria:macrophage ratio of 50:1) at 37 degrees C for 90 min. Supernatant eicosanoids were extracted and separated by reverse phase high performance liquid chromatography (RP-HPLC). While the amounts of labelled PGE2, TXB2, and PGD2 produced in response to the two organisms were equal, the amount of PGF2 alpha elicited by S. aureus amounted to three times that released during macrophage challenge with P. aeruginosa. Overall, preferential release of cyclooxygenase products occurred during phagocytosis of S. aureus. In contrast, eicosanoids identified presumptively as oxygenated metabolites of AA predominated in cultures challenged with opsonized P. aeruginosa. Live, non-opsonized P. aeruginosa elicited the same profile of eicosanoids, but in reduced amounts. Inhibitor studies indicated that these AA derivatives were not synthesized via the macrophage lipoxygenase pathway. Their production was dependent on ...Continue Reading

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Citations

Jul 16, 2008·Marine Biotechnology·Steven RobertsFrederick Goetz
Aug 7, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Myungsoo JooJohn W Christman
May 1, 1992·Infection and Immunity·T C SorrellK Sztelma

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