Eicosapentaenoic acid and docosahexaenoic acid effects on tumour mitochondrial metabolism, acyl CoA metabolism and cell proliferation

Cell Biochemistry and Function
A ColquhounR I Schumacher

Abstract

In order to investigate the effects of high-fat diets rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), Wistar rats bearing subcutaneous implants of the Walker 256 tumour were fed pelleted chow containing low DHA/EPA or high DHA/EPA. The presence of n-3 polyunsaturated fatty acids (PUFAs) led to a marked suppression (35-46%) of tumour growth over a 12 day period. Both the whole tumour homogenate and the Percoll-purified mitochondrial fraction presented significant changes in fatty acid composition. The levels of EPA increased in both n-3 dietary groups while the levels of DHA increased only in the high DHA/EPA group, in comparison with the control chow-fed group. The presence of n-3 PUFAs led to an increase in mitochondrial acyl CoA synthetase activity, but neither the cytoplasmic acyl CoA content nor the n-3 fatty acid composition of the cytoplasmic acyl CoAs was altered by the diet. The content of thiobarbituric acid-reactive substances (TBARS) was increased in the low DHA/EPA group but was unchanged in the high DHA/EPA group. In vitro studies with the Walker 256 cell line showed a 46% decrease in cell growth in the presence of either EPA or DHA which was accompanied by a large decrease in the measured mitoc...Continue Reading

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Citations

Apr 30, 2010·Molecular Neurobiology·Alison Colquhoun
Nov 20, 2013·Chemico-biological Interactions·Béatrice DendeléDominique Lagadic-Gossmann
Sep 20, 2007·Lipids in Health and Disease·Brian M RossLee E Sieswerda
Jun 5, 2004·Biochemical and Biophysical Research Communications·Shengjun Qiao, Pentti Tuohimaa
Dec 15, 2005·International Journal of Cancer. Journal International Du Cancer·Magnus LindskogJohn Inge Johnsen
Jan 2, 2007·Cell Biochemistry and Function·Renata GorjãoRui Curi
Dec 13, 2007·American Journal of Physiology. Endocrinology and Metabolism·Sarah L WakefieldMegan Mitchell

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