Eicosapentaenoic acid and docosahexaenoic acid modulate mitogen-activated protein kinase activity in endothelium

Vascular Pharmacology
Hua XueJieshou Li

Abstract

Omega-3 polyunsaturated fatty acids (PUFA) regulate inflammation and immunoreaction partially via affecting endothelial functions. However, the intracellular signaling mechanisms for inhibiting endothelial activation by omega-3 PUFA remain unclear. We investigated the effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on mitogen-activated protein kinases (MAPK) of endothelium. We analyzed the expression of extracellular signal-related kinases (ERK1/2), Jun amino-terminal kinases (JNK), and p38 mRNA by real-time RT-PCR and the kinases activity by western blotting in tumor necrosis factor-alpha (TNF-alpha)-activated human umbilical vein endothelial cells (HUVEC). We observed that EPA or DHA alone significantly reduced the TNF-alpha-induced activation of p38 and JNK kinases at a concentration of 20 microM, but EPA is a more potent inhibitor than DHA. In contrast, both EPA and DHA significantly counteracted the TNF-alpha-mediated deactivation of ERK1/2 kinases. Meanwhile, both EPA and DHA significantly attenuated the TNF-alpha-induced expression of p38 and ERK1/2 mRNA, and DHA but not EPA also reduced the TNF-alpha-induced JNK mRNA expression. We present data show that both EPA and DHA alone diminish activation o...Continue Reading

References

Apr 1, 1990·Physiological Reviews·J S Pober, R S Cotran
Feb 1, 1995·Prostaglandins, Leukotrienes, and Essential Fatty Acids·R De CaterinaP Libby
Jan 3, 1997·Biochemical and Biophysical Research Communications·A PietersmaW Sluiter
Mar 17, 1997·Biochemical and Biophysical Research Communications·Y OkudaK Yamashita
Aug 12, 1998·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·P C Calder
Jun 13, 2000·JPEN. Journal of Parenteral and Enteral Nutrition·C J LoS Helton
Jan 22, 2001·The Journal of Biological Chemistry·B MirnikjooE J Weeber
Mar 7, 2001·Circulation Research·T TakahashiS Kitagawa
Mar 10, 2001·The Journal of Biological Chemistry·H ZhangS Pelech
Mar 10, 2001·Nature·L Chang, M Karin
May 17, 2001·Proceedings of the National Academy of Sciences of the United States of America·J SurapisitchatB C Berk
Mar 16, 2002·The British Journal of Nutrition·H GrimmE Eigenbrodt
Jul 12, 2002·Arthritis Research·Jordan S Pober
Jul 19, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Konstantin MayerWerner Seeger
Dec 14, 2002·Journal of the American College of Nutrition·Artemis P Simopoulos
Dec 19, 2002·Vascular Pharmacology·Ryan J Hoefen, Bradford C Berk
Feb 27, 2003·The Journal of Experimental Biology·Kyra J Cowan, Kenneth B Storey
Jan 16, 2004·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Koichi InoueMasatoshi Hagiwara
Apr 9, 2004·The Proceedings of the Nutrition Society·Parveen Yaqoob
Feb 8, 2005·The Journal of Clinical Investigation·Hideyuki YamawakiBradford C Berk

❮ Previous
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Citations

Sep 10, 2013·International Journal of Cardiology·Vincenzo MollaceFrancesca Oppedisano
Sep 12, 2013·The Proceedings of the Nutrition Society·Cherry L Wainwright, Lisa Michel
Aug 6, 2008·Apoptosis : an International Journal on Programmed Cell Death·Simona SeriniGabriella Calviello
Apr 19, 2011·The Journal of Nutritional Biochemistry·Silvia Lorente-CebriánMaria J Moreno-Aliaga
Mar 10, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Xiuqin KongMei Li
Mar 26, 2009·Pharmacology & Therapeutics·Renata GorjãoRui Curi
Oct 26, 2007·Brain Research Reviews·Akhlaq A FarooquiTahira Farooqui
Sep 9, 2010·Molecular Nutrition & Food Research·Jae Young ChoHyang Sook Chun
Aug 30, 2011·European Journal of Pharmacology·Osamu TakaseToshiro Fujita
May 16, 2012·Free Radical Biology & Medicine·Pitchai Balakumar, Gaurav Taneja
Mar 9, 2010·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Robert Ringseis, Klaus Eder
Jun 3, 2011·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Ayman IbrahimRachel Marion-Letellier
Oct 2, 2014·Archives of Pharmacal Research·Byoung-Kook JeonYeun-Ja Mun
Nov 10, 2018·Frontiers in Microbiology·Abel Ramos-VegaCarlos Angulo
Feb 13, 2021·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Youjia DuPeter Zahradka

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