eIF4E Phosphorylation Influences Bdnf mRNA Translation in Mouse Dorsal Root Ganglion Neurons

Frontiers in Cellular Neuroscience
Jamie K MoyTheodore J Price

Abstract

Plasticity in dorsal root ganglion (DRG) neurons that promotes pain requires activity-dependent mRNA translation. Protein synthesis inhibitors block the ability of many pain-promoting molecules to enhance excitability in DRG neurons and attenuate behavioral signs of pain plasticity. In line with this, we have recently shown that phosphorylation of the 5' cap-binding protein, eIF4E, plays a pivotal role in plasticity of DRG nociceptors in models of hyperalgesic priming. However, mRNA targets of eIF4E phosphorylation have not been elucidated in the DRG. Brain-derived neurotrophic factor (BDNF) signaling from nociceptors in the DRG to spinal dorsal horn neurons is an important mediator of hyperalgesic priming. Regulatory mechanisms that promote pain plasticity via controlling BDNF expression that is involved in promoting pain plasticity have not been identified. We show that phosphorylation of eIF4E is paramount for Bdnf mRNA translation in the DRG. Bdnf mRNA translation is reduced in mice lacking eIF4E phosphorylation (eIF4ES209A ) and pro-nociceptive factors fail to increase BDNF protein levels in the DRGs of these mice despite robust upregulation of Bdnf-201 mRNA levels. Importantly, bypassing the DRG by giving intrathecal inje...Continue Reading

References

Oct 17, 1980·European Journal of Pharmacology·J L Hylden, G L Wilcox
Jul 1, 1994·Journal of Neuroscience Methods·S R ChaplanT L Yaksh
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·R J MannionC J Woolf
Jan 18, 2006·Molecular and Cellular Neurosciences·Jing ZhaoUNKNOWN London Pain Consortium
Jun 17, 2006·Annual Review of Neuroscience·Sophie Pezet, Stephen B McMahon
Sep 4, 2007·Biochemical and Biophysical Research Communications·Yoshikazu MatsuokaTohru Nakanishi
Oct 9, 2007·Molecular and Cellular Neurosciences·C ChiaruttiniE Tongiorgi
Oct 19, 2007·Neurobiology of Learning and Memory·Yuan LuBai Lu
Jun 21, 2008·Experimental Neurology·Li-Jun ZhouXian-Guo Liu
Aug 4, 2010·Proceedings of the National Academy of Sciences of the United States of America·Luc FuricNahum Sonenberg
Nov 12, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ohannes K MelemedjianTheodore J Price
Feb 8, 2011·Journal of Medicinal Chemistry·Scott BoitanoJosef Vagner
Mar 24, 2011·Nature Reviews. Molecular Cell Biology·Armen ParsyanNahum Sonenberg
Apr 7, 2011·Biochemical and Biophysical Research Communications·Norihiko ObataMasataka Yokoyama
Feb 15, 2012·Journal of Neuroscience Research·Kathleen M SalernoKathryn M Albers
May 4, 2012·Nature·Carson C ThoreenDavid M Sabatini
Nov 23, 2012·Nature·Christos G GkogkasNahum Sonenberg
Aug 1, 2013·Proceedings of the National Academy of Sciences of the United States of America·Kateryna FeoktistovaChristopher S Fraser
Mar 13, 2014·The European Journal of Neuroscience·Wenling ChenJuan Carlos G Marvizón
Aug 1, 2014·Nature·Andrew L WolfeHans-Guido Wendel
Nov 21, 2014·Nature·Feng YueUNKNOWN Mouse ENCODE Consortium
Jan 23, 2015·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Maja GenhedenChristopher G Proud
Apr 29, 2015·Nature Neuroscience·Ruifeng CaoNahum Sonenberg
May 9, 2015·Science·Marta MeléRoderic Guigó
Jul 5, 2017·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jamie K MoyTheodore J Price
Oct 28, 2017·Pain Medicine : the Official Journal of the American Academy of Pain Medicine·Theodore J Price, Michael S Gold
Dec 19, 2017·Trends in Neurosciences·Arkady Khoutorsky, Theodore J Price

❮ Previous
Next ❯

Citations

Oct 8, 2019·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Stephanie ShiersTheodore J Price
Sep 24, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Sandra M MihailTheodore J Price
Sep 24, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·S M Géranton
Jul 3, 2020·GeroScience·Prapti H ModyMichael D Burton
Feb 9, 2019·Nature Reviews. Endocrinology·Adilson GuilhermeMichael P Czech
Nov 22, 2018·Frontiers in Genetics·Sonali UttamArkady Khoutorsky
Nov 19, 2020·Pharmacological Reviews·Muhammad Saad YousufTheodore J Price
Mar 31, 2021·The World Journal of Biological Psychiatry : the Official Journal of the World Federation of Societies of Biological Psychiatry·Chih-Yu TsaoLi-Jen Lee
Apr 30, 2021·Frontiers in Immunology·Nilesh M AgalaveMichael D Burton

❮ Previous
Next ❯

Methods Mentioned

BETA
protein assay

Software Mentioned

mfold
Image Lab
ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved