Abstract
Hxt2 and Hxt1 are high affinity and low affinity facilitative glucose transporter paralogs of Saccharomyces cerevisiae, respectively, that differ at 75 amino acid positions in their 12 transmembrane segments (TMs). Comprehensive analysis of chimeras of these two proteins has previously revealed that TMs 1, 5, 7, and 8 of Hxt2 are required for high affinity glucose transport activity and that leucine 201 in TM5 is the most important in this regard of the 20 amino acid residues in these regions that differ between Hxt2 and Hxt1. To evaluate the importance of the remaining residues, we systematically shuffled the amino acids at these positions and screened the resulting proteins for high affinity and high capacity glucose transport activity. In addition to leucine 201 (TM5), four residues of Hxt2 (leucine 59 and leucine 61 in TM1, asparagine 331 in TM7, and phenylalanine 366 in TM8) were found to be important for such activity. Furthermore, phenylalanine 198 (TM5), alanine 363 (TM8), and either valine 316 (TM7) or alanine 368 (TM8) were found to be supportive of maximal activity. Construction of a homology model suggested that asparagine 331 interacts directly with the substrate and that the other identified residues may contribut...Continue Reading
References
Feb 10, 1995·The Journal of Biological Chemistry·K NishizawaM Kasahara
Nov 11, 1994·Nucleic Acids Research·J D ThompsonT J Gibson
Jun 8, 1993·Biochimica Et Biophysica Acta·S A Baldwin
Apr 1, 1996·The Biochemical Journal·T Kasahara, M Kasahara
Jul 1, 1996·FEBS Letters·M KasaharaM Maeda
Nov 1, 1996·Archives of Microbiology·A L Kruckeberg
Jul 4, 1997·The Journal of Biological Chemistry·M KasaharaM Maeda
Aug 1, 1997·FEMS Microbiology Reviews·E Boles, C P Hollenberg
Apr 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·S S PaoM H Saier
Jul 27, 1999·Journal of Bacteriology·L YeK van Dam
Feb 8, 2000·The Journal of Biological Chemistry·T Kasahara, M Kasahara
Feb 20, 2003·Journal of Bacteriology·Teruhisa HiraiSriram Subramaniam
Feb 27, 2003·The Biochemical Journal·Toshiko Kasahara, Michihiro Kasahara
Aug 2, 2003·Science·Jeff AbramsonSo Iwata
Aug 2, 2003·Science·Yafei HuangDa-Neng Wang
May 7, 2004·The Journal of Biological Chemistry·Toshiko KasaharaMichihiro Kasahara
Jun 25, 2004·Protein Science : a Publication of the Protein Society·Eyal VardyShimon Schuldiner
Aug 25, 2004·Biophysical Journal·Alexis Salas-BurgosJorge Fischbarg
Apr 13, 2005·Biochemistry·Janet M WoodRobert A B Keates
Sep 28, 2005·The Journal of Biological Chemistry·Andrei ManolescuChris I Cheeseman
Citations
Jan 27, 2010·Plant Molecular Biology·Daniel Padilla-ChacónEleazar Martínez-Barajas
Sep 7, 2011·Biochemistry·Toshiko KasaharaMichihiro Kasahara
Jun 8, 2010·The Journal of Biological Chemistry·Toshiko Kasahara, Michihiro Kasahara
Jul 21, 2014·Enzyme and Microbial Technology·Davi L GonçalvesBoris U Stambuk
Apr 18, 2015·Journal of Applied Microbiology·O ReznicekB Hauer
Sep 16, 2016·Frontiers in Microbiology·Kang-Wei LinPeng Cai
Nov 23, 2018·Journal of Applied Microbiology·D TrichezB U Stambuk
Mar 27, 2019·The EMBO Journal·Timo StahlAnne Spang
Mar 21, 2007·The Journal of Biological Chemistry·Toshiko KasaharaMichihiro Kasahara
Dec 15, 2017·Journal of Applied Microbiology·J G NijlandA J M Driessen
Apr 5, 2018·Scientific Reports·Evert BosdrieszBas Teusink
Nov 7, 2007·Biochemistry·Navneet K TyagiRolf K H Kinne
Apr 23, 2021·Chemical Reviews·David DrewMikio Tanabe