Eight-cell parthenotes originated from in vitro grown sheep preantral follicles

Reproductive Sciences
V B LuzJ R Figueiredo

Abstract

We investigated the effect of the leukemia inhibitory factor (LIF) alone or in association with follicle-stimulating hormone (FSH) on the in vitro growth and antrum formation of sheep preantral follicles. To evaluate oocyte quality, parthenogenetic activation of the oocytes recovered from in vitro grown preantral follicles was performed. Preantral follicles >110 μm in diameter were isolated and cultured for 18 days in basic medium either alone (control) or supplemented with LIF (10 or 50 ng/mL) in the absence or presence of FSH. Every 6 days the follicular survival, growth, and antrum formation were evaluated. When compared to control (P < .05), antrum formation was increased in follicles cultured in the presence of LIF10 and FSH. At the end of the culture, the oocytes underwent in vitro maturation (IVM); their viability and chromatin configuration were assessed. Although IVM was not affect by the treatments (P > .05), the numerically highest maturation rates (29.63%) were obtained when follicles were cultured in 50 ng/mL LIF (LIF50). Therefore, their oocytes were submitted to parthenogenetic activation; from which 58.3% of the mature oocytes resulted in 8-cell stage parthenotes. In conclusion, although LIF10 + FSH increases an...Continue Reading

References

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Citations

Feb 3, 2016·Domestic Animal Endocrinology·J R V SilvaJ R Figueiredo
May 8, 2015·Cell and Tissue Research·Franciele Osmarini LunardiAna Paula Ribeiro Rodrigues
Apr 11, 2017·Biopreservation and Biobanking·Franciele Osmarini LunardiAna Paula Ribeiro Rodrigues
Feb 13, 2019·The Journal of Reproduction and Development·Yasuhisa MunakataHisataka Iwata
May 31, 2019·Reproduction in Domestic Animals = Zuchthygiene·Gildas Tetaping MbemyaAna Paula Ribeiro Rodrigues
Jun 22, 2021·Cell and Tissue Banking·Francisco Denilson Rodrigues GomesAna Paula Ribeiro Rodrigues
Aug 15, 2014·Reproductive Biology and Endocrinology : RB&E·Valdevane R AraújoEduardo L Gastal

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Methods Mentioned

BETA
fluorescence microscopy

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