PMID: 3320891Dec 1, 1987Paper

Eimeria tenella sporozoites: the method of excystation affects the surface membrane proteins

Parasitology
M H Wisher, M E Rose

Abstract

Eimerian sporozoites can be recovered from intestinal washings after oral administration of oocysts to chickens but suspensions of sporozoites are usually prepared in the laboratory by incubation of sporocysts or fractured oocysts in vitro, at body temperatures, with relatively high concentrations of trypsin and bile salts. Since these agents affect membrane structure, the surface membrane of proteins of Eimeria tenella sporozoites excysted in vivo and in vitro have been compared. Surface radio-iodination followed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showed that more 125I was incorporated into polypeptides on sporozoites excysted in vivo than on sporozoites excysted in vitro. The 125I-polypeptide profile of sporozoites excysted in vivo was more resistant to subsequent incubation with pure trypsin than that of sporozoites excysted in vitro, but incubation with bile salts resulted in the loss of some iodinated polypeptides from both preparations of iodinated sporozoites. Reaction with combinations of crude trypsin and bile salts led to the lysis of sporozoites. The method of excystation had no effect on the reaction of convalescent chicken serum with Western blots of sporozoites but the results...Continue Reading

References

Jul 4, 1978·Zeitschrift Für Parasitenkunde·H D Chapman
Mar 25, 1975·Biochimica Et Biophysica Acta·A Helenius, K Simons
Oct 1, 1986·Molecular and Biochemical Parasitology·M H Wisher
Jan 20, 1984·Journal of Immunological Methods·S M Freer
May 1, 1962·The Journal of protozoology·D J DORAN, M M FARR
Nov 1, 1962·The Journal of protozoology·M M FARR, D J DORAN
Apr 1, 1955·Biochimica Et Biophysica Acta·G W SCHWERT, Y TAKENAKA

❮ Previous
Next ❯

Citations

Oct 1, 1993·Veterinary Immunology and Immunopathology·A RhalemP Péry
Aug 1, 1990·Veterinary Parasitology·B RobertP Coppe
Sep 28, 2007·Veterinary Immunology and Immunopathology·Daad Abi-GhanemLuc R Berghman
Jul 1, 1989·The Journal of protozoology·A J TreesS D Carter
Jun 25, 1999·Biochimica Et Biophysica Acta·M V NesterenkoS J Upton
Oct 1, 1993·Infection and Immunity·P S DoyleC Petersen

❮ Previous
Next ❯

Related Concepts

Related Feeds