Ejaculate-hormonal traits in the leopard cat (Felis bengalensis) and sperm function as measured by in vitro penetration of zona-free hamster ova and zona-intact domestic cat oocytes

Molecular Reproduction and Development
J Howard, D Wildt

Abstract

Electroejaculate traits and circulating follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone concentrations were analyzed in adult leopard cats (Felis bengalensis), a rare felid species indigenous to east Asia. The ability of leopard cat sperm to bind and penetrate zona-free hamster ova and zona-intact domestic cat oocytes in vitro was examined as a means of testing sperm function. The influence of culture media [Biggers, Whitten, Whittingham (BWW) vs. modified Krebs Ringer bicarbonate (mKRB)], seminal plasma removal, and swim-up separation on sperm motility, sperm morphology, and oocyte penetration also were assessed. Sperm treatments included dilution of raw semen (DR), ejaculate centrifugation, and either resuspension (NS) or swim-up processing (SU). The percentage of oocytes penetrated (penetration rate) and the number of penetrated sperm/oocyte (penetration index) were determined. Ejaculates from each male consisted of at least a 50% sperm motility rating, and hormone concentrations in individual males were unrelated to any ejaculate trait measured concurrently on the same day. The SU technique improved (P less than 0.05) percent sperm motility and the proportion of structurally normal sperm compa...Continue Reading

References

Jul 1, 1989·Journal of Reproduction and Fertility·A P ByersR L Tilson
Oct 1, 1989·The Journal of Experimental Zoology·K L GoodroweD E Wildt
Sep 1, 1988·Biology of Reproduction·K L GoodroweD E Wildt
Aug 1, 1987·Journal of in Vitro Fertilization and Embryo Transfer : IVF·M M MahadevanJ F Leeton
May 1, 1985·The Journal of Endocrinology·K L GoodroweD E Wildt
Jun 1, 1985·Fertility and Sterility·B J Rogers
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Mar 1, 1972·Journal of Reproduction and Fertility·R Yanagimachi
Dec 1, 1964·Experimental Cell Research·P E LINDAHL, L O DREVIUS
Jul 29, 1983·Science·S J O'brienM Bush

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Citations

Aug 23, 2001·Theriogenology·V K HarnalD E Wildt
Sep 2, 2003·Journal of Feline Medicine and Surgery·G C LuvoniG Marinoni
Aug 25, 2009·Reproduction, Fertility, and Development·Natalia GañánEduardo R S Roldan

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