EjAP2-1, an AP2/ERF gene, is a novel regulator of fruit lignification induced by chilling injury, via interaction with EjMYB transcription factors

Plant Biotechnology Journal
Jiao-ke ZengKunsong Chen

Abstract

Lignin biosynthesis is regulated by many transcription factors, such as those of the MYB and NAC families. However, the roles of AP2/ERF transcription factors in lignin biosynthesis have been rarely investigated. Eighteen EjAP2/ERF genes were isolated from loquat fruit (Eriobotrya japonica), which undergoes postharvest lignification during low temperature storage. Among these, expression of EjAP2-1, a transcriptional repressor, was negatively correlated with fruit lignification. The dual-luciferase assay indicated that EjAP2-1 could trans-repress activities of promoters of lignin biosynthesis genes from both Arabidopsis and loquat. However, EjAP2-1 did not interact with the target promoters (Ej4CL1). Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays indicated protein-protein interactions between EjAP2-1 and lignin biosynthesis-related EjMYB1 and EjMYB2. Furthermore, repression effects on the Ej4CL1 promoter were observed with the combination of EjAP2-1 and EjMYB1 or EjMYB2, while EjAP2-1 with the EAR motif mutated (mEjAP2-1) lost such repression, although mEjAP2-1 still interacted with EjMYB protein. Based on these results, it is proposed that EjAP2-1 is an indirect transcriptional repressor on l...Continue Reading

References

Apr 1, 1998·Bioinformatics·T L Bailey, M Gribskov
Dec 17, 2003·The Plant Journal : for Cell and Molecular Biology·Astrid PatzlaffMalcolm M Campbell
Dec 24, 2003·The Plant Journal : for Cell and Molecular Biology·Lisa J NewmanMalcolm M Campbell
Mar 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Fernando NovilloJulio Salinas
Oct 8, 2004·The Plant Journal : for Cell and Molecular Biology·Michael WalterJörg Kudla
Jan 13, 2006·Plant Physiology·Toshitsugu NakanoHideaki Shinshi
Oct 24, 2007·Current Opinion in Plant Biology·Ruiqin Zhong, Zheng-Hua Ye
Feb 19, 2008·Trends in Plant Science·Andrew C AllanWilliam A Laing
Dec 19, 2008·Plant Physiology·Olivia WilkinsMalcolm M Campbell
Oct 20, 2009·Plant Signaling & Behavior·Ruiqin Zhong, Zheng-Hua Ye
Nov 4, 2009·Journal of Experimental Botany·Ping WangKunsong Chen
Feb 2, 2010·The Plant Journal : for Cell and Molecular Biology·Francesco LicausiPierdomenico Perata
May 12, 2010·Plant Physiology·Xue-Ren YinIan B Ferguson
May 18, 2010·Plant Physiology·Ruben VanholmeWout Boerjan
Dec 15, 2010·The Plant Journal : for Cell and Molecular Biology·Mi-Young ChungJames Giovannoni
Jan 6, 2011·Plant Physiology·Madana M R AmbavaramAndy Pereira
Jan 14, 2011·Trends in Plant Science·Qiao Zhao, Richard A Dixon
Sep 20, 2011·Journal of Experimental Botany·Jian-fei KuangWang-jin Lu
Dec 31, 2011·The Plant Cell·Rashmi Sasidharan, Angelika Mustroph
Oct 1, 2004·The New Phytologist·Louisa A Rogers, Malcolm M Campbell

❮ Previous
Next ❯

Citations

Jul 14, 2016·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Darren Chern Jan WongJosé Tomás Matus
Mar 17, 2017·Plant Biotechnology Journal·Miao-Miao WangKun-Song Chen
Feb 3, 2018·Journal of Experimental Botany·Kaixuan ZhangMeiliang Zhou
Apr 26, 2016·PloS One·Wen-Qiu WangKun-Song Chen
Jun 11, 2019·Frontiers in Plant Science·Jianghua CaiShiping Tian
Mar 9, 2017·Frontiers in Plant Science·Ujjal J PhukanRakesh K Shukla
May 14, 2018·Plant Biotechnology Journal·Cheng XueJun Wu
Aug 3, 2019·Trends in Plant Science·Marc BehrMarie Baucher
Jun 3, 2021·International Journal of Molecular Sciences·Danqi ZengJuan Duan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.