EjTFL1 Genes Promote Growth but Inhibit Flower Bud Differentiation in Loquat

Frontiers in Plant Science
Yuanyuan JiangShunquan Lin

Abstract

TERMINAL FLOWER1 (TFL1), a key factor belonging to the phosphatidyl ethanolamine-binding protein (PEBP) family, controls flowering time and inflorescence architecture in some plants. However, the role of TFL1 in loquat remains unknown. In this study, we cloned two TFL1-like genes (EjTFL1-1 and EjTFL1-2) with conserved deduced amino acid sequences from cultivated loquat (Eriobotrya japonica Lindl.). First, we determined that flower bud differentiation occurs at the end of June and early July, and then comprehensively analyzed the temporal and spatial expression patterns of these EjTFL1s during loquat growth and development. We observed the contrasting expression trends for EjTFL1s and EjAP1s (APETALA 1) in shoot apices, and EjTFL1s were mainly expressed in young tissues. In addition, short-day and exogenous GA3 treatments promoted the expression of EjTFL1s, and no flower bud differentiation was observed after these treatments in loquat. Moreover, EjTFL1s were localized to the cytoplasm and nucleus, and both interacted with another flowering transcription factor, EjFD, in the nucleus, and EjTFL1s-EjFD complex significantly repressed the promoter activity of EjAP1-1. The two EjTFL1s were overexpressed in wild-type Arabidopsis thal...Continue Reading

References

May 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yoshie HanzawaDesmond Bradley
Jun 14, 2005·Plant & Cell Physiology·Ayako YamaguchiTakashi Araki
Oct 3, 2007·Genes & Development·Yasushi Kobayashi, Detlef Weigel
Jan 15, 2008·The Plant Cell·Raili RuonalaChristiaan van der Schoot
Jan 22, 2008·Plant & Cell Physiology·Tomohiro IgasakiNobuhiro Kotoda
Apr 22, 2008·Nature Cell Biology·Susana Ubeda-TomásMalcolm J Bennett
Aug 7, 2009·The Plant Journal : for Cell and Molecular Biology·Seonghoe JangGeorge Coupland
Mar 6, 2010·The Plant Journal : for Cell and Molecular Biology·Rozi MohamedAmy M Brunner
Apr 3, 2010·Science·Kerstin KaufmannJosé Luis Riechmann
Oct 16, 2010·Trends in Genetics : TIG·Frank Wellmer, José L Riechmann
Sep 8, 2011·The Plant Journal : for Cell and Molecular Biology·Hikaru IwataFabrice Foucher
Mar 10, 2012·Trends in Plant Science·Nathalie GonzalezDirk Inzé
Apr 12, 2012·Development·Li Yen Candy LeeHao Yu
Jun 19, 2012·The Plant Journal : for Cell and Molecular Biology·Nien-Chen HuangTien-Shin Yu
Aug 18, 2012·Nature Reviews. Genetics·Fernando Andrés, George Coupland
Dec 4, 2012·Plant Science : an International Journal of Experimental Plant Biology·Ravit Goldberg-MoellerAvi Sadka
Aug 10, 2013·Journal of Experimental Botany·Takeshi KurokuraTimo Hytönen
Feb 18, 2014·The Plant Cell·William Wing Ho Ho, Detlef Weigel
Jun 20, 2015·Plant Science : an International Journal of Experimental Plant Biology·Yohei Higuchi, Tamotsu Hisamatsu
Jun 28, 2016·Current Opinion in Plant Biology·Hilde NelissenDirk Inzé

❮ Previous
Next ❯

Methods Mentioned

BETA
two-hybrid
PCR
Y2H
Fluorescence
fluorescence microscopy
Assay
transgenic

Software Mentioned

MEGA
Clustal X
Gene Doc

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.