Elaboration and validation of the method for the quantification of the emetic toxin of Bacillus cereus as described in EN-ISO 18465 - Microbiology of the food chain - Quantitative determination of emetic toxin (cereulide) using LC-MS/MS

International Journal of Food Microbiology
P in 't VeldElisabeth G Biesta-Peters

Abstract

A method for the quantification of the Bacillus cereus emetic toxin (cereulide) was developed and validated. The method principle is based on LC-MS as this is the most sensitive and specific method for cereulide. Therefore the study design is different from the microbiological methods validated under this mandate. As the method had to be developed a two stage validation study approach was used. The first stage (pre-study) focussed on the method applicability and the experience of the laboratories with the method. Based on the outcome of the pre-study and comments received during voting at CEN and ISO level a final method was agreed to be used for the second stage the (final) validation of the method. In the final (validation) study samples of cooked rice (both artificially contaminated with cereulide or contaminated with B. cereus for production of cereulide in the rice) and 6 other food matrices (fried rice dish, cream pastry with chocolate, hotdog sausage, mini pancakes, vanilla custard and infant formula) were used. All these samples were spiked by the participating laboratories using standard solutions of cereulide supplied by the organising laboratory. The results of the study indicate that the method is fit for purpose. R...Continue Reading

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Citations

Nov 27, 2018·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Hiroshi KoikeTsuneo Hashimoto
Sep 11, 2019·Food Microbiology·Katia Rouzeau-SzynalskiMonika Ehling-Schulz
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Mariateresa FeroneAmalia G M Scannell
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Andreja RajkovicMieke Uyttendaele
Feb 3, 2021·Toxins·Richard DietrichPer Einar Granum
Jun 24, 2021·Comprehensive Reviews in Food Science and Food Safety·Jelena JovanovicAndreja Rajkovic
Jun 27, 2021·Letters in Applied Microbiology·Yong YangLingling Mei

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