Elastase inhibition affects collagen transcription and prostaglandin secretion in mare endometrium during the estrous cycle

Reproduction in Domestic Animals = Zuchthygiene
A AmaralG Ferreira-Dias

Abstract

We have shown that bacteria induce neutrophil extracellular traps (NETs) in mare endometrium. Besides killing pathogens, NETs may contribute for endometrosis (chronic endometrium fibrosis). Since elastase (ELA) is a NETs component that regulates fibrosis and prostaglandin (PG) output, the aim was to evaluate if inhibition of ELA would affect collagen 1 (COL1) transcription and PGs secretion by endometrium explants, in different estrous cycle phases. Follicular-FP (n = 8) and mid luteal-MLP (n = 7) phases explants were cultured for 24-48 hr with medium alone (Control), ELA (0.5 μg/ml,1 μg/ml), sivelestat - ELA inhibitor (INH,10 μg/ml), or ELA (0.5 μg/ml,1 μg/ml) + INH (10 μg/ml). COL1 gene transcription was done by qRT-PCR and PGE2 and PGF2 α determination in culture medium by EIA. In FP, at 24 hr, ELA0.5 increased COL1 transcription (p < 0.001) but its inhibition (ELA0.5 + INH10) decreased COL1 transcription (p < 0.01) and PGF2 α production (p < 0.05). Also, ELA0.5 + INH10 or ELA1 + INH10 raised PGE2 production (p < 0.01). At 48 hr, ELA1 increased COL1 transcription (p < 0.01) and PGF2 α production (p < 0.001), but its inhibition (ELA1 + INH10) decreased these actions (p < 0.01; p < 0.05, respectively). Besides, ELA1 + INH10 in...Continue Reading

References

Mar 6, 2004·Science·Volker BrinkmannArturo Zychlinsky
Dec 8, 2009·Nature Medicine·Mitchell A Olman
Nov 17, 2010·Pharmacological Reviews·Brice KorkmazFrancis Gauthier
Dec 1, 2010·Molecular Human Reproduction·Wolfgang R SchäferHans P Zahradnik
Mar 23, 2011·American Journal of Respiratory Cell and Molecular Biology·Paul D Bozyk, Bethany B Moore
Mar 24, 2012·The European Respiratory Journal·Akihiro TakemasaTakeshi Fukuda
Apr 18, 2014·The Journal of Pathology·Akrivi ChrysanthopoulouKonstantinos Kambas
Sep 15, 2014·Journal of Reproductive Immunology·M R RebordãoG Ferreira-Dias
Mar 7, 2015·Journal of Leukocyte Biology·Alyssa D GregoryA McGarry Houghton
Sep 12, 2015·Bioorganic & Medicinal Chemistry Letters·Franz von Nussbaum, Volkhart M-J Li
Feb 18, 2018·Theriogenology·Maria Rosa RebordãoGraça Ferreira-Dias

❮ Previous
Next ❯

Citations

May 21, 2020·Animals : an Open Access Journal From MDPI·Ana AmaralGraça Ferreira-Dias
Nov 17, 2020·Frontiers in Veterinary Science·Ana AmaralGraça Ferreira-Dias
Jan 25, 2020·Theriogenology·Dariusz J SkarzynskiGraça M Ferreira-Dias
Jan 21, 2021·Animals : an Open Access Journal From MDPI·Ana AmaralGraça Ferreira-Dias
Jun 3, 2021·International Journal of Molecular Sciences·Ana AmaralGraça Ferreira-Dias

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antitubercular Agents (ASM)

Antitubercular agents are pharmacologic agents for treatment of tuberculosis. Discover the latest research on antitubercular agents here.

Antitubercular Agents

Antitubercular agents are pharmacologic agents for treatment of tuberculosis. Discover the latest research on antitubercular agents here.