Abstract
We have shown that bacteria induce neutrophil extracellular traps (NETs) in mare endometrium. Besides killing pathogens, NETs may contribute for endometrosis (chronic endometrium fibrosis). Since elastase (ELA) is a NETs component that regulates fibrosis and prostaglandin (PG) output, the aim was to evaluate if inhibition of ELA would affect collagen 1 (COL1) transcription and PGs secretion by endometrium explants, in different estrous cycle phases. Follicular-FP (n = 8) and mid luteal-MLP (n = 7) phases explants were cultured for 24-48 hr with medium alone (Control), ELA (0.5 μg/ml,1 μg/ml), sivelestat - ELA inhibitor (INH,10 μg/ml), or ELA (0.5 μg/ml,1 μg/ml) + INH (10 μg/ml). COL1 gene transcription was done by qRT-PCR and PGE2 and PGF2 α determination in culture medium by EIA. In FP, at 24 hr, ELA0.5 increased COL1 transcription (p < 0.001) but its inhibition (ELA0.5 + INH10) decreased COL1 transcription (p < 0.01) and PGF2 α production (p < 0.05). Also, ELA0.5 + INH10 or ELA1 + INH10 raised PGE2 production (p < 0.01). At 48 hr, ELA1 increased COL1 transcription (p < 0.01) and PGF2 α production (p < 0.001), but its inhibition (ELA1 + INH10) decreased these actions (p < 0.01; p < 0.05, respectively). Besides, ELA1 + INH10 in...Continue Reading
References
Mar 6, 2004·Science·Volker BrinkmannArturo Zychlinsky
May 2, 2006·Domestic Animal Endocrinology·R P Roberto da CostaD J Skarzynski
Dec 8, 2009·Nature Medicine·Mitchell A Olman
Nov 17, 2010·Pharmacological Reviews·Brice KorkmazFrancis Gauthier
Dec 1, 2010·Molecular Human Reproduction·Wolfgang R SchäferHans P Zahradnik
Mar 23, 2011·American Journal of Respiratory Cell and Molecular Biology·Paul D Bozyk, Bethany B Moore
Aug 23, 2011·Theriogenology·J LehmannH-A Schoon
Mar 24, 2012·The European Respiratory Journal·Akihiro TakemasaTakeshi Fukuda
Sep 28, 2013·PloS One·Anthony J O'DonoghueTerry W Hermiston
Apr 18, 2014·The Journal of Pathology·Akrivi ChrysanthopoulouKonstantinos Kambas
Sep 15, 2014·Journal of Reproductive Immunology·M R RebordãoG Ferreira-Dias
Mar 7, 2015·Journal of Leukocyte Biology·Alyssa D GregoryA McGarry Houghton
Sep 12, 2015·Bioorganic & Medicinal Chemistry Letters·Franz von Nussbaum, Volkhart M-J Li
Feb 18, 2018·Theriogenology·Maria Rosa RebordãoGraça Ferreira-Dias
Citations
May 21, 2020·Animals : an Open Access Journal From MDPI·Ana AmaralGraça Ferreira-Dias
Feb 26, 2020·International Journal of Molecular Sciences·Igor F CanissoCarleigh E Fedorka
Nov 17, 2020·Frontiers in Veterinary Science·Ana AmaralGraça Ferreira-Dias
Jul 3, 2019·Cytokine·A Z Szóstek-MioduchowskaD J Skarzynski
Jan 25, 2020·Theriogenology·Dariusz J SkarzynskiGraça M Ferreira-Dias
Jan 21, 2021·Animals : an Open Access Journal From MDPI·Ana AmaralGraça Ferreira-Dias
Jun 3, 2021·International Journal of Molecular Sciences·Ana AmaralGraça Ferreira-Dias