Elastic coupling power stroke mechanism of the F1 -ATPase molecular motor

Proceedings of the National Academy of Sciences of the United States of America
James MartinWayne D Frasch

Abstract

The angular velocity profile of the 120° F1-ATPase power stroke was resolved as a function of temperature from 16.3 to 44.6 °C using a ΔμATP = -31.25 kBT at a time resolution of 10 μs. Angular velocities during the first 60° of the power stroke (phase 1) varied inversely with temperature, resulting in negative activation energies with a parabolic dependence. This is direct evidence that phase 1 rotation derives from elastic energy (spring constant, κ = 50 kBT·rad-2). Phase 2 of the power stroke had an enthalpic component indicating that additional energy input occurred to enable the γ-subunit to overcome energy stored by the spring after rotating beyond its 34° equilibrium position. The correlation between the probability distribution of ATP binding to the empty catalytic site and the negative Ea values of the power stroke during phase 1 suggests that this additional energy is derived from the binding of ATP to the empty catalytic site. A second torsion spring (κ = 150 kBT·rad-2; equilibrium position, 90°) was also evident that mitigated the enthalpic cost of phase 2 rotation. The maximum ΔGǂ was 22.6 kBT, and maximum efficiency was 72%. An elastic coupling mechanism is proposed that uses the coiled-coil domain of the γ-subunit...Continue Reading

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Citations

Feb 2, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hendrik SielaffMichael Börsch
Sep 12, 2019·Proceedings of the National Academy of Sciences of the United States of America·Wonmuk Hwang, Martin Karplus
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Jun 14, 2020·Mitochondrion·Marco ZancaniGiovanna Lippe

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