Elastic reversible valves on centrifugal microfluidic platforms

Lab on a Chip
Mohammad Mahdi AeinehvandSergio O Martínez-Chapa

Abstract

Reversible valves on centrifugal microfluidic platforms facilitate the automation of bioanalytical assays, especially of those requiring a series of steps (such as incubation) in a single reaction chamber. In this study, we present fixed elastic reversible (FER) valves and tunable elastic reversible (TER) valves that are easy to fabricate, implement and control. In the FER valve the compression of an elastic barrier/patch against a microchamber's outlet prevents the release of liquid. The valve sealing pressure was determined by adjusting the engraving depth of the valve-seat at which the elastic patch was located, this allows to set the sealing pressure during disc fabrication. In the TER valve, the patch compression value and sealing pressure is controlled by the penetration depth of a plastic screw into the valve-seat. The ER valves prevent liquid flow until the centrifugal force overcomes their sealing pressure. Moreover, at a constant spin speed, turning the screw of a TER valve reduces its sealing pressure and opens the valve. Therefore, the TER valve allows for controlling of the liquid transfer volume at various spin speeds. The FER and TER valves' behavior is mathematically described and equations for the prediction of...Continue Reading

References

Aug 2, 2008·Angewandte Chemie·Volker StadlerFrank Breitling
Mar 5, 2011·Lab on a Chip·Hyundoo HwangYoon-Kyoung Cho
Jul 24, 2013·Scientific Reports·Wang XuYoung-Tae Chang
Jan 21, 2014·Lab on a Chip·Mohammad Mahdi AeinehvandMarc Madou
Mar 7, 2014·Advanced Materials·Frieder MaerkleAlexander Nesterov-Mueller
Jun 3, 2015·Chemical Society Reviews·O StrohmeierN Paust
Jun 30, 2015·Analytical Chemistry·Yubin KimYoon-Kyoung Cho
Jul 15, 2015·Lab on a Chip·Mohammad Mahdi AeinehvandMarc Madou
Jun 15, 2016·Nature Communications·Felix F LoefflerAlexander Nesterov-Mueller
Aug 19, 2016·Lab on a Chip·Tae-Hyeong KimYoon-Kyoung Cho
Feb 9, 2017·Journal of Immunological Methods·Laura K WeberFelix F Loeffler
Sep 19, 2017·Biotechnology Journal·Andrea PalermoFrank Breitling
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Jul 20, 2017·Engineering in Life Sciences·Laura K WeberFelix F Loeffler

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Citations

Oct 10, 2019·Lab on a Chip·J F HessN Paust
Oct 28, 2019·Sensors·Sean C MorganVincent J Sieben
Sep 15, 2020·Biospektrum : Zeitschrift Der Gesellschaft Für Biologishe Chemie (GBCH) Und Der Vereinigung Für Allgemeine Und Angewandte Mikrobiologie (VAAM)·Fabian FalkFrank Breitling
May 4, 2021·ACS Sensors·Thilanga N PahattugeSteven A Soper
Jul 31, 2021·Journal of Healthcare Engineering·Yuxing ShiWenhao Zhao
Nov 11, 2020·Analytical Chemistry·Simon F BerlandaPetra S Dittrich
Nov 14, 2019·Analytical Chemistry·Jacob B NielsenAdam T Woolley

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Methods Mentioned

BETA
antibody
peptide array

Software Mentioned

PEPperPRINT
M3
PepSlide Analyzer

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