Elasticity Modulation of Fibroblast-Derived Matrix for Endothelial Cell Vascular Morphogenesis and Mesenchymal Stem Cell Differentiation

Tissue Engineering. Part a
Ping DuKangwon Lee

Abstract

Biophysical properties of the microenvironment, including matrix elasticity and topography, are known to affect various cell behaviors; however, the specific role of each factor is unclear. In this study, fibroblast-derived matrix (FDM) was used as cell culture substrate and physically modified to investigate the influence of its biophysical property changes on human umbilical vein endothelial cells (HUVECs) and human mesenchymal stem cells (hMSCs) behavior in vitro. These FDMs were physically modified by simply storing them at different temperatures: the one stored at 4°C, maintained its original properties, was considered natural FDM, whereas the ones stored at -20°C or -80°C, exhibited a distinct surface morphology, were considered physically modified FDM. Physical modification induced matrix fiber rearrangement in FDM, forming different microstructures on the surface as characterized by focused ion beam (FIB)-cryoSEM. A significant increase of matrix elasticity was found with physically modified FDMs as determined by atomic force microscopy. HUVEC and hMSC behaviors on these natural and physically modified FDMs were observed and compared with each other and with gelatin-coated coverslips. HUVECs showed a similar adhesion le...Continue Reading

References

Sep 17, 2005·Journal of Cellular Biochemistry·Song LiSteven Hsu
Nov 19, 2005·Science·Dennis E DischerYu-Li Wang
Feb 14, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Victor W M van HinsberghPaul H A Quax
Aug 23, 2006·Cell·Adam J EnglerDennis E Discher
Mar 31, 2007·Circulation Research·Laurent LamaliceJacques Huot
Aug 9, 2007·Colloids and Surfaces. B, Biointerfaces·Zuwei MaChangyou Gao
Nov 30, 2007·Biophysical Journal·Jérôme SolonPaul A Janmey
Feb 27, 2009·Nature·Akiko MammotoDonald E Ingber
Apr 21, 2009·Matrix Biology : Journal of the International Society for Matrix Biology·Patricia A Soucy, Lewis H Romer
Jan 29, 2010·Annals of Biomedical Engineering·Ramji T VenkatasubramanianJohn C Bischof
Apr 20, 2010·Biomaterials·Sara J LiliensiekChristopher J Murphy
Sep 24, 2010·American Journal of Physiology. Cell Physiology·Ramaswamy KrishnanGeerten P van Nieuw Amerongen
Oct 30, 2010·Nature Protocols·Ashok Kumar, Akshay Srivastava
Dec 15, 2010·Proceedings of the National Academy of Sciences of the United States of America·Xuanhe ZhaoDavid J Mooney
Mar 2, 2011·Cellular and Molecular Bioengineering·Kimberly M Stroka, Helim Aranda-Espinoza
Mar 1, 2010·Cellular and Molecular Bioengineering·Randi L Saunders, Daniel A Hammer
Sep 20, 2011·Cell·Michael PotentePeter Carmeliet
Nov 30, 2011·Drug Discovery Today. Disease Models·George E Davis
Jan 27, 2012·American Journal of Physiology. Cell Physiology·Abigail C HielscherSharon Gerecht
May 25, 2013·American Journal of Physiology. Cell Physiology·Britta DreierChristopher J Murphy
Aug 12, 2014·Nature Materials·Jessica H WenAdam J Engler
Feb 7, 2015·ACS Applied Materials & Interfaces·Hyeona JeonDeok-Ho Kim
Jul 8, 2015·ACS Applied Materials & Interfaces·Guobao ChenYonggang Lv
Feb 14, 2007·Soft Matter·Ilya LeventalPaul A Janmey

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Citations

Jul 18, 2017·Molecular Medicine Reports·Xueqin WangXiangyu Zhou

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Methods Mentioned

BETA
atomic force microscopy
bio-AFM
AFM
PCR
Assay

Software Mentioned

JPK
Image J
GraphPad Prism
Imaris

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