Elastin-like polypeptide and γ-zein fusions significantly increase recombinant protein accumulation in soybean seeds.

Transgenic Research
Jing YangXiangdong Yang

Abstract

Soybean seeds are an ideal host for the production of recombinant proteins because of their high content of proteins, long-term stability of seed proteins under ambient conditions, and easy establishment of efficient purification protocols. In this study, a polypeptide fusion strategy was applied to explore the capacity of elastin-like polypeptide (ELP) and γ-zein fusions in increasing the accumulation of the recombinant protein in soybean seeds. Transgenic soybean plants were generated to express the γ-zein- or ELP-fused green fluorescent protein (GFP) under the control of the soybean seed-specific promoter of β-conglycinin alpha subunit (BCSP). Significant differences were observed in the accumulation of zein-GFP and GFP-ELP from that of the unfused GFP in transgenic soybean seeds based on the total soluble protein (TSP), despite the low-copy of T-DNA insertions and similar expression at the mRNA levels in selected transgenic lines. The average levels of zein-GFP and GFP-ELP accumulated in immature seeds of these transgenic lines were 0.99% and 0.29% TSP, respectively, compared with 0.07% TSP of the unfused GFP. In mature soybean seeds, the accumulation of zein-GFP and GFP-ELP proteins was 1.8% and 0.84% TSP, an increase of 3...Continue Reading

References

Oct 27, 2004·Plant Physiology·Davide MainieriAlessandro Vitale
Jun 10, 2008·Journal of Experimental Botany·Maddalena de VirgilioAlessandro Vitale
Jul 22, 2008·Biotechnology and Bioengineering·A R KusnadiJ A Howard
Jan 30, 2009·BMC Biology·Margarita TorrentM Dolors Ludevid
May 23, 2009·Biotechnology Advances·On Sun Lau, Samuel S M Sun
Nov 10, 2009·Trends in Biotechnology·Doreen M FlossJürgen Scheller
May 27, 2010·Plant Biotechnology Journal·Joseph BootheMaurice M Moloney
Nov 12, 2010·Transgenic Research·Nicolau B CunhaElíbio L Rech
Nov 12, 2010·Transgenic Research·Nicolau B CunhaElíbio L Rech
Apr 13, 2011·Plant Biotechnology Journal·Francesca MorandiniMario Pezzotti
Aug 25, 2011·Genetics and Molecular Research : GMR·G R ViannaE L Rech
Nov 2, 2011·Proceedings of the National Academy of Sciences of the United States of America·Yang HeDaichang Yang
Mar 20, 2012·BMC Plant Biology·Minu JosephJean-Benoit Peltier
Jan 3, 2014·BMC Genomics·Eun-Young HwangPerry B Cregan
May 14, 2014·BioMed Research International·Laura C HudsonKenneth J Piller
Jun 1, 2016·Frontiers in Plant Science·Reza SaberianfarRima Menassa
Mar 24, 2017·Plant Biotechnology Journal·Reza Saberianfar, Rima Menassa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.