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Elastin-like polypeptides as a purification tag for recombinant proteins

Current Protocols in Protein Science

Sep 4, 2010

Wafa HassounehA Chilkoti

Abstract

This unit presents a recombinant protein purification method that employs an elastin-like polypeptide (ELP) as a purification tag. ELPs undergo a sharp and reversible phase transition when heated above their lower critical solution temperature. ELPs retain this behavior when they are fu...read more

Mentioned in this Paper

ELN gene
Alkalescens-Dispar Group
Chimeric Proteins, Recombinant
Chromatography
Particle Size
Staphylococcal Protein A
Soluble
Isolate compound
Protein Purification
Purification Aspects
Paper Details
References
  • References25
  • Citations12
123
  • References25
  • Citations12
12

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Elastin-like polypeptides as a purification tag for recombinant proteins

Current Protocols in Protein Science

Sep 4, 2010

Wafa HassounehA Chilkoti

PMID: 20814933

DOI: 10.1002/0471140864.ps0611s61

Abstract

This unit presents a recombinant protein purification method that employs an elastin-like polypeptide (ELP) as a purification tag. ELPs undergo a sharp and reversible phase transition when heated above their lower critical solution temperature. ELPs retain this behavior when they are fu...read more

Mentioned in this Paper

ELN gene
Alkalescens-Dispar Group
Chimeric Proteins, Recombinant
Chromatography
Particle Size
Staphylococcal Protein A
Soluble
Isolate compound
Protein Purification
Purification Aspects

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Purification of recombinant proteins by fusion with thermally-responsive polypeptides

Nature BiotechnologyNovember 5, 1999
D E Meyer, A Chilkoti
Paper Details
References
  • References25
  • Citations12
123
  • References25
  • Citations12
12
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