PMID: 3745952Mar 1, 1986Paper

Elastin production in human skin fibroblast cultures and its decline with age

The Journal of Investigative Dermatology
G C Sephel, J M Davidson

Abstract

Recent studies have established that cultured human skin fibroblasts secrete the soluble precursor of elastin, tropoelastin (TE). The present studies evaluate, by an enzyme-linked immunosorbent assay, the stability of the TE phenotype and the effect of culture conditions and donor age on TE accumulation by human skin fibroblasts. Tropoelastin was maximally produced by 2 control fibroblast strains at early confluency (32-49 X 10(3) molecules/cell/h), and its serum-dependent accumulation in the medium was linear for at least 72 h. Inhibition of cross-linking had no effect on the rate of elastin production. Optimum serum concentrations for TE production differed for fibroblast cell strains derived from foreskin and trunk skin fibroblasts. Production of TE by human skin fibroblasts was stable through nearly 30 population doublings after which there was a greater than 2-fold decline in the rate of accumulation. In a cohort of donor strains, TE production appeared to decline at donor ages greater than or equal to 70 years. Under standard culture conditions, cell strains from normal donors of various ages produced TE at rates ranging from 25-69 X 10(3) molecules/cell/h. Rates of TE accumulation in medium were not significantly altered...Continue Reading

References

Jan 1, 1979·Connective Tissue Research·G QuintarelliR P Mecham
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P A JonesW Gevers
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jul 1, 1979·The Journal of Investigative Dermatology·W Montagna, K Carlisle
Dec 1, 1979·Archives of Biochemistry and Biophysics·R Eichner, J Rosenbloom
Aug 23, 1977·Biochemistry·R P Mecham, J A Foster
Mar 1, 1976·The Journal of Cell Biology·A S NarayananD L Layman
Oct 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·E L Schneider, Y Mitsui
Jan 5, 1976·Biochimica Et Biophysica Acta·B FarisC Franzblau
Sep 1, 1985·Archives of Biochemistry and Biophysics·K YoonE Golub
Jul 15, 1985·The Biochemical Journal·K S Cheah
Jun 4, 1974·Biochemical and Biophysical Research Communications·P A AbrahamW H Carnes
Nov 10, 1970·Biochemistry·R C SiegelG R Martin
Jan 1, 1971·Analytical Biochemistry·R T Hinegardner
Aug 9, 1984·The New England Journal of Medicine·D J Prockop, K I Kivirikko
Sep 1, 1983·Journal of Cellular Physiology·R P MechamU Ryan
Jan 1, 1981·Connective Tissue Research·L B SandbergB W Oakes
Mar 1, 1980·Cell Biology International Reports·M C BourdillonL Robert
May 1, 1982·The Journal of Investigative Dermatology·I M Braverman, E Fonferko
Mar 1, 1981·Journal of Cellular Physiology·D R Clemmons, J J Van Wyk
Nov 1, 1980·The Journal of Investigative Dermatology·H FreibergerS R Pinnell

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Citations

Jan 1, 1995·Archives of Dermatological Research·S TajimaT Nishikawa
Oct 26, 1999·The Journal of the Acoustical Society of America·R W Chan, I R Titze
May 7, 2014·Journal of Tissue Engineering and Regenerative Medicine·Partha Pratim Deb, Anand Ramamurthi
Oct 17, 2019·Cold Spring Harbor Perspectives in Medicine·Mitchell Fane, Ashani T Weeraratna
Apr 5, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Barry StarcherCharles H Hill
May 28, 2020·Plastic and Reconstructive Surgery·Luiz Charles-de-SáRadovan Borojevic
May 27, 2020·International Journal of Cosmetic Science·K KennedyN Khaldi
Oct 1, 1990·The Journal of Dermatologic Surgery and Oncology·M Yaar, B A Gilchrest
Jan 29, 2013·Drug Delivery and Translational Research·Balakrishnan SivaramanAnand Ramamurthi
Oct 13, 2016·International Journal of Cosmetic Science·A C WeihermannC M de Carvalho
Jun 1, 2017·Scientific Reports·Zheng-Mei XiongKan Cao
Dec 15, 2019·Nature Reviews. Cancer·Mitchell Fane, Ashani T Weeraratna
Dec 5, 2006·Skin Pharmacology and Physiology·K R MewesR Graf
Aug 31, 2019·Scientific Reports·Rabab SharifDimitrios Karamichos
Jul 30, 2020·Stem Cells International·Luiz Charles-de-SáGino Rigotti
Aug 15, 1997·Microscopy Research and Technique·I Pasquali-Ronchetti, M Baccarani-Contri
Jan 12, 2021·Biomaterials Science·Xifang ChenGordon G Wallace
Mar 16, 2021·The Journal of Clinical Investigation·Asurayya WorredeAshani T Weeraratna
Jul 28, 2020·Journal of Photochemistry and Photobiology. B, Biology·Zejun ZhangChangzhi Dong
Jan 29, 2000·The Journal of Investigative Dermatology·F Duplan-PerratF Braye
Mar 1, 1991·Mutation Research·C S EleftheriouS J Tzartos
Aug 15, 1993·Mechanisms of Ageing and Development·M Giro, J M Davidson
Jan 1, 1988·Experimental Gerontology·R J Shmookler Reis
Apr 1, 1989·The Journal of Investigative Dermatology·J UittoM J Fazio
Jul 1, 1989·The Journal of Investigative Dermatology·G C SephelJ M Davidson
May 1, 1988·The Journal of Investigative Dermatology·G C SephelJ M Davidson
Jun 1, 1987·The Journal of Investigative Dermatology·G C SephelJ M Davidson
Aug 1, 1992·The Journal of Investigative Dermatology·M G GiroJ M Davidson

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