PMID: 6169729Aug 1, 1981Paper

Elastin synthesis by ligamentum nuchae fibroblasts: effects of culture conditions and extracellular matrix on elastin production

The Journal of Cell Biology
R P MechamB Starcher

Abstract

Fetal bovine ligamentum nuchae fibroblasts maintained in culture synthesized soluble elastin but were unable to form the insoluble elastic fiber. Secreted elastin precursors accumulated in culture medium and were measured using a radioimmunoassay for elastin. When elastin production was examined in ligament tissue from fetal calves of various gestational ages, cells from tissue taken during the last trimester of development produced significantly more elastin than did cells from younger fetal tissue, with maximal elastin synthesis occurring shortly before birth. Soluble elastin was detected in ligament cells plated at low density until proliferation began to be density inhibited and the cells became quiescent. Also, soluble elastin production per cell declined with increasing population doubling or with age in culture. Cells grown in the presence of 5% fetal calf serum produced approximately four times as much soluble elastin as cells grown in serum-free medium. The addition of dexamethasone (0.1 microM) and bleomycin (1 microgram/ml) increased soluble elastin production by cultured cells 180% and 50%, respectively, whereas theophylline (5 micrograms/ml) depressed production 50% and antagonized stimulation by dexamethasone. Asc...Continue Reading

References

Sep 23, 1977·Biochemical and Biophysical Research Communications·B C StarcherA S Tepper
Aug 1, 1975·FEBS Letters·B T Hill, S Whatley
Nov 1, 1979·Biochimica Et Biophysica Acta·J A FosterG Lange
Dec 1, 1979·Archives of Biochemistry and Biophysics·R Eichner, J Rosenbloom
Jun 1, 1978·Cell·L B ChenM L Walsh
Jan 1, 1978·Journal of Immunological Methods·S L Cookson, D O Adams
Oct 1, 1976·Experimental Cell Research·R SchwarzP Doty
Mar 1, 1976·The Journal of Cell Biology·A S NarayananD L Layman
May 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·R MayneE J Miller
Jul 1, 1966·The Journal of Cell Biology·T K GreenleeJ L Hartman
Apr 1, 1972·The Journal of Experimental Medicine·G GabbianiG Majno
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·A B Pardee
Jun 4, 1974·Biochemical and Biophysical Research Communications·P A AbrahamW H Carnes
Jun 1, 1967·The Journal of Cell Biology·E G ClearyD S Jackson
Jan 1, 1980·Connective Tissue Research·R P Mecham, G Lange
Jul 1, 1964·The Journal of Cell Biology·B GOLDBERG, H GREEN

❮ Previous
Next ❯

Citations

May 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·K A LeeW C Parks
Jul 1, 1990·Journal of Cellular Biochemistry·A R JohnsonJ Sullivan
Dec 2, 2009·Annals of Biomedical Engineering·E Jane Walter, Sarah M Wells
Sep 1, 1985·Journal of Ultrastructure Research·A Jaques, A Serafini-Fracassini
Apr 1, 1986·Journal of Ultrastructure and Molecular Structure Research·A Jaques, A Serafini-Fracassini
Sep 28, 1998·Matrix Biology : Journal of the International Society for Matrix Biology·E C Davis, R P Mecham
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J D MaloneA J Kahn
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·L FouserP Bornstein
Oct 19, 2011·Rejuvenation Research·Giulia AnnoviDaniela Quaglino
Jan 10, 2012·Tissue Engineering. Part B, Reviews·Chris A BashurAnand Ramamurthi
Nov 17, 1999·Molecular Biology of the Cell·B W RobbE C Davis
Oct 1, 1983·Pediatric Dermatology·K A Holbrook
Oct 1, 1989·The American Review of Respiratory Disease·E C CrouchR P Mecham
Sep 1, 1982·The Journal of Clinical Investigation·R M SeniorR P Mecham
Mar 1, 1986·The Journal of Clinical Investigation·R M SeniorG D Wilner
Apr 1, 1984·Environmental Health Perspectives·R B Rucker, M A Dubick
Jan 1, 1982·Cancer Metastasis Reviews·P A Jones, Y A De Clerck
Jan 29, 2013·Drug Delivery and Translational Research·Balakrishnan SivaramanAnand Ramamurthi
Jan 1, 1981·Connective Tissue Research·R P Mecham
Jan 1, 1982·Connective Tissue Research·J Rosenbloom
Jan 1, 1983·Connective Tissue Research·S J SkinnerG C Liggins
Jan 1, 1984·Connective Tissue Research·K MuramotoR Stern
Jan 1, 1990·Connective Tissue Research·D J Johnson, F W Keeley
Jun 1, 1995·The Journal of Pathology·L E Grosso, D L Lacey
Sep 15, 1990·International Journal of Cancer. Journal International Du Cancer·C CaenazzoS Garbisa
Jul 1, 1990·Matrix : Collagen and Related Research·M C Sutcliffe, J M Davidson
Mar 1, 1993·Matrix : Collagen and Related Research·L E Grosso, M Scott
Oct 31, 2012·Birth Defects Research. Part C, Embryo Today : Reviews·Jessica F AlmineAnthony S Weiss
Aug 9, 2005·Pathologie-biologie·Ladislas Robert
May 1, 1984·The Journal of Surgical Research·S BarkA A Demetriou
Feb 1, 1988·Journal of Cellular Physiology·A J Millis, M Hoyle
Sep 1, 1983·Journal of Cellular Physiology·R P MechamU Ryan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.