PMID: 7537379Apr 25, 1995Paper

Electrical and synaptic properties of embryonic luteinizing hormone-releasing hormone neurons in explant cultures

Proceedings of the National Academy of Sciences of the United States of America
K KusanoSusan Wray

Abstract

Voltage- and ligand-activated channels in embryonic neurons containing luteinizing hormone-releasing hormone (LHRH) were studied by patch-pipette, whole-cell current and voltage clamp techniques. LHRH neurons were maintained in explant cultures derived from olfactory pit regions of embryonic mice. Cells were marked intracellularly with Lucifer yellow following recording. Sixty-two cells were unequivocally identified as LHRH neurons by Lucifer yellow and LHRH immunocytochemistry. The cultured LHRH neurons had resting potentials around -50 mV, exhibited spontaneous discharges generated by intrinsic and/or synaptic activities and contained a time-dependent inward rectifier (Iir). Voltage clamp analysis of ionic currents in the LHRH neuron soma revealed a tetrodotoxin-sensitive Na+ current (INa) and two major types of K+ currents, a transient current (IA), a delayed rectifier current (IK) and low- and high-voltage-activated Ca2+ currents. Spontaneous depolarizing synaptic potentials and depolarizations induced by direct application of gamma-aminobutyrate were both inhibited by picrotoxin or bicuculline, demonstrating the presence of functional gamma-aminobutyrate type A synapses on these neurons. Responses to glutamate were found i...Continue Reading

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Citations

Mar 1, 1996·Trends in Endocrinology and Metabolism : TEM·P S Tsai, R I Weiner
May 24, 2003·Frontiers in Neuroendocrinology·Suzanne M MoenterCraig S Nunemaker
Dec 12, 2001·Molecular and Cellular Endocrinology·S A TobetG A Schwarting
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Jun 18, 2010·Endocrinology·Stephanie ConstantinSusan Wray
Sep 15, 2012·Journal of Cell Science·Filippo CasoniSusan Wray
Dec 21, 2006·The Journal of Reproduction and Development·Hitomi FujiokaMasugi Nishihara
Oct 12, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Zhiguo ChuSuzanne M Moenter
Aug 28, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Chunguang ZhangOline K Rønnekleiv
Jan 14, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Paolo E ForniSusan Wray
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Mar 23, 2017·Endocrinology·Yanlong SongWei Hu
Jul 20, 2002·Frontiers in Neuroendocrinology·S Wray

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