Electrical field-assisted solid-phase extraction coupled on-line to capillary electrophoresis-mass spectrometry

Electrophoresis
Gabriel Morales-CidMiguel Valcárcel

Abstract

A substantial demand currently exists for analytical methods affording the determination of very low concentrations of analytes in complex matrices, such as those of environmental and biological samples, as simply as possible. However, the pretreatment of complex samples, which is unavoidable prior to CE-MS analysis, is usually complicated and time-consuming. In this work, we used voltage-assisted SPE for the first time as an alternative to conventional treatments for preconcentrating and purifying analytes. To this end, we used a simple flow system coupled on-line to CE-MS equipment. The system is quite robust and provides reproducible peak areas (the precision ranges from 2.5 to 3.8%). Also, it provides increased sensitivity affording the determination of trace amounts (nanogram per liter levels) of analytes in only a few milliliters of sample. The proposed system was applied to the determination of members of two compound families (viz. tetracyclines and amines).

References

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Apr 12, 2007·Electrophoresis·Bricio SantosMiguel Valcárcel

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Citations

Jun 18, 2009·Analytical and Bioanalytical Chemistry·Ana M García-CampañaCarmen Cruces-Blanco
Apr 21, 2009·Electrophoresis·Virginia Rodríguez Robledo, William Franklin Smyth
Apr 21, 2012·Journal of Separation Science·Mi-Hung Wang, Shu-Ping Wang
Dec 10, 2009·Electrophoresis·María Castro-PuyanaMaría Luisa Marina
Dec 17, 2009·Electrophoresis·Rawi RamautarGerhardus J de Jong
Dec 6, 2014·Talanta·Shahram SeidiMaryam Rezazadeh
May 21, 2010·Analytical Chemistry·Douglas E Raynie
May 26, 2010·Analytical Chemistry·Nicholas W FrostMichael T Bowser

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