Electrical properties of gap junction channels in guinea-pig ventricular cell pairs revealed by exposure to heptanol.

Pflügers Archiv : European journal of physiology
A Rüdisüli, R Weingart

Abstract

Cell pairs were isolated from adult guinea pig ventricles to study the electrical properties of gap junction channels. The experiments involved a double voltage-clamp approach and whole-cell, tight-seal recording. Heptanol decreased the intracellular current, In, in a dose-dependent fashion. Before complete uncoupling, In showed fluctuations suggesting the operation of gated channels. In the presence of 3 mM heptanol, In showed quantal steps arising from spontaneous opening and closing of single channels. The IV-relationship of the channels was linear (range: +/- 95 mV). Analysis of current records revealed the following single-channel conductances, gamma n: Mean value = 37 pS; median value = 33 pS. gamma n was insensitive to the non-junctional membrane potential (range: -90 to +10 mV). 3 mM ATP4- in the pipette solution had no effects on gamma n, 6 mM ATP4- produced a small decrease, and 6 mM ATP + 0.1 mM cAMP- an increase in gamma n. Channel transitions from closed to open state were variable (range of apparent time constants: 2.5-32 ms; mean: 11 ms).

References

Apr 25, 1978·Pflügers Archiv : European journal of physiology·W Trautwein, T F McDonald
Sep 30, 1976·Pflügers Archiv : European journal of physiology·G Isenberg
Sep 2, 1985·Nature·J Neyton, A Trautmann
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D C SprayE L Hertzberg
Aug 29, 1986·Science·R D Veenstra, R L DeHaan
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P MedaL Orci
Oct 1, 1987·The Journal of Physiology·N M Cohen, W J Lederer
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J M Burt, D C Spray
Feb 1, 1989·The American Journal of Physiology·E NiggliR Weingart
Aug 1, 1987·Pflügers Archiv : European journal of physiology·P Maurer, R Weingart
Jan 1, 1984·Annual Review of Physiology·B Sakmann, E Neher
Jul 31, 1980·Nature·M F JohnstonF Ramón
Jun 1, 1982·The Journal of General Physiology·R S KassK J Malloy
Sep 1, 1980·Archives of Biochemistry and Biophysics·R L KaoE H Williams

❮ Previous
Next ❯

Citations

Oct 1, 1990·Pflügers Archiv : European journal of physiology·G S FluriR Weingart
Jan 1, 1995·Pflügers Archiv : European journal of physiology·B BastideJ Délèze
May 1, 1993·Pflügers Archiv : European journal of physiology·P N Münster, R Weingart
Apr 1, 1993·Pflügers Archiv : European journal of physiology·F F Bukauskas, R Weingart
Jan 6, 2012·The Journal of Physiological Sciences : JPS·Gary TseChristopher L-H Huang
Sep 1, 1991·Mathematical Biosciences·C R MurpheyW R Giles
Mar 1, 1992·Trends in Cardiovascular Medicine·J E SaffitzE C Beyer
Aug 1, 1990·The Journal of Cell Biology·G I FishmanL A Leinwand
Mar 4, 2008·Journal of Neurosurgery·Tao HongHuan Wang
Feb 1, 1997·Journal of Cardiovascular Electrophysiology·J F SpearE N Moore
Dec 1, 1996·Journal of Cardiovascular Electrophysiology·D J CallansJ F Spear
Aug 1, 1994·Cell Adhesion and Communication·R M MègeF Rieger
Apr 2, 2015·Journal of Molecular and Cellular Cardiology·Karin P HammerDonald M Bers
Aug 2, 2003·Journal of Cardiovascular Electrophysiology·Bum-Rak ChoiGuy Salama
May 1, 2013·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Sonya M Bierbower, Robin L Cooper
Feb 2, 2011·Biophysical Journal·Kunichika TsumotoYoshihisa Kurachi
Apr 1, 1991·Circulation Research·W L Nelson, J C Makielski
Sep 1, 1991·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·P D Drumheller, J A Hubbell
Dec 24, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Andrew E Pollard, Roger C Barr
Mar 6, 2016·American Journal of Physiology. Heart and Circulatory Physiology·Sharon A GeorgeSteven Poelzing
Jun 1, 1992·Pflügers Archiv : European journal of physiology·T SchettinoI Fucile
Jun 1, 1992·The American Journal of Physiology·B R Takens-KwakA C Van Ginneken
Nov 13, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Guy LagaudIsmail Laher
Aug 1, 1994·The American Journal of Physiology·W A Varanda, A C de Carvalho
Feb 1, 1994·The American Journal of Physiology·M ChansonD C Spray
Jul 16, 2021·The Journal of General Physiology·Nicolae MoiseSeth H Weinberg

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.