May 1, 1982

Electrical properties of the basolateral membrane of the straight portion of the rabbit proximal renal tubule

The Journal of Physiology
E Bello-Reuss


1. Cell membrane potentials were measured with intracellular 3 M-CKl microelectrodes in isolated, perfused segments of the straight portion of the rabbit proximal tubule. 2. Under in vitro conditions simulating the in vivo situation, the transepithelial potential difference was about 1.6 mV, lumen-positive, and the basolateral membrane potential was 61 mV, cell negative. 3. Isomolar single ion substitutions in the bath (K+ for Na+, isethionate for Cl-, N-methyl-D-glucamine (NMDG+) for Na+, and Cl- for HCO3-) resulted in significant basolateral membrane potential changes only when [K+] was increased and [HCO3-] was reduced; in both cases the basolateral membrane depolarized. Cl- and Na+ substitutions with large monovalent ions did not change basolateral membrane potential. 4. Transepithelial potential changes in substitution experiments suggest that, at the paracellular pathway, PK greater than PNa greater than PNMDG, and PCl greater than Pisethionate. 5. It is concluded that the basolateral membrane of these cells is mainly K+-conductive and that electrodiffusional PNa and PCl are undetectable by this technique. 6. Addition of 1 mM-Ba2+ to the bath reduced basolateral membrane electro-diffusional PK, as evidenced by depolarizat...Continue Reading

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Mentioned in this Paper

Chloride Ion Level
Biochemical Pathway
Entire Lumen of Body System
Resting Potentials
Carbonic Acid Ions

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