PMID: 1214284Dec 4, 1975Paper

Electrical properties of the cellular transepithelial pathway in Necturus gallbladder. II. Ionic permeability of the apical cell membrane

The Journal of Membrane Biology
L Reuss, A L Finn

Abstract

Microelectrode techniques were employed to study the ionic permeability of the apical cell membrane of Necturus gallbladder epithelium. Results obtained from continuous records in single cells, and from several cellular impalements shortly after a change in solution, were similar and indicate that both the apical membrane equivalent electromotive force (Va) and electrical resistance (Ra) strongly depend on external [K]. Cl substitutions produced smaller effects, while the effects of Na substitutions with N-methyl-D-glucamine on both Va and Ra were minimal. These results indicate that the permeability sequence of the apical membrane is PKgreater thanPClgreater than PNa. From the calculated absolute value of PNa it is possible to estimate the diffusional Na flux from the mucosal solution into the cells (from the cell potential and an assumed intracellular Na concentration). The calculated flux is roughly three orders of magnitude smaller than the measured net transepithelial flux in this tissue and in gallbladders of other species. Thus, only a minimal portion of Na entry can be attributed to independent diffusion. From estimations of the electrochemical potential gradient across the apical membrane, Cl transport at that site mus...Continue Reading

References

Jun 1, 1975·The Journal of General Physiology·R A FrizzellS G Schultz
Jan 1, 1975·Pflügers Archiv : European journal of physiology·S Hénin, D Cremaschi
Jan 1, 1974·The Journal of Membrane Biology·J G SpenneyG Sachs
Jan 5, 1972·Nature: New Biology·E Frömter, J Diamond
Jan 1, 1972·The Journal of Membrane Biology·E Frömter
Mar 24, 1971·Nature: New Biology·P J FritzK M Pruitt
Aug 1, 1967·The Journal of General Physiology·A Strickholm, B G Wallin
Mar 1, 1961·The Journal of General Physiology·G WHITTEMBURYA K SOLOMON
May 1, 1962·The Journal of Physiology·J M DIAMOND
May 1, 1962·The Journal of Physiology·J M DIAMOND
Oct 1, 1959·The Journal of Physiology·A L HODGKIN, P HOROWICZ
Mar 3, 1949·The Journal of Physiology·A L HODGKIN, B KATZ

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Citations

Jan 1, 1990·Pflügers Archiv : European journal of physiology·C P Hansen, O Frederiksen
Jun 1, 1986·Pflügers Archiv : European journal of physiology·Y MaruyamaT Hoshi
Mar 28, 1979·The Journal of Membrane Biology·N K WillsD C Eaton
Jun 7, 1979·The Journal of Membrane Biology·J Graf, G Giebisch
Jan 1, 1983·The Journal of Membrane Biology·S R ShorofskyH A Fozzard
Jan 1, 1983·The Journal of Membrane Biology·J Fernando Garcia-DiazW M Armstrong
May 1, 1988·The Journal of Membrane Biology·J S Stoddard, L Reuss
Jan 1, 1984·The Journal of Membrane Biology·R S Fisher, K R Spring
Dec 15, 1977·The Journal of Membrane Biology·R C Rose, D L Nahrwold
Dec 15, 1977·The Journal of Membrane Biology·L Reuss, A L Finn
Jun 28, 1978·The Journal of Membrane Biology·S A LewisD C Eaton
Jan 1, 1987·The Journal of Membrane Biology·P S ReinachG Klemperer
Jan 1, 1981·The Journal of Membrane Biology·K U PetersenK Heintze
Jan 1, 1983·The Journal of Membrane Biology·A Corcia, W M Armstrong
Mar 1, 1987·Pflügers Archiv : European journal of physiology·W WangH Oberleithner
Jan 1, 1986·Comparative Biochemistry and Physiology. A, Comparative Physiology·M Acevedo, A Diez de los Rios
Dec 1, 1992·Comparative Biochemistry and Physiology. Comparative Physiology·D Cremaschi, C Porta
Aug 1, 1979·Biophysical Journal·A M Weinstein, J L Stephenson
Sep 1, 1983·Biophysical Journal·J F García-DíazA Essig
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·L Reuss
Mar 1, 1979·The Journal of General Physiology·K R Spring, A Hope
May 1, 1983·The Journal of General Physiology·K U Petersen, L Reuss
Mar 1, 1985·The Journal of General Physiology·L Reuss, K U Petersen
May 1, 1991·The Journal of General Physiology·C U Cotton, L Reuss
Feb 1, 1992·The Journal of General Physiology·G A AltenbergL Reuss
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·C G Vanoye, L Reuss
Jan 1, 1982·The Journal of Membrane Biology·H J BaerentsenT Zeuthen
Jan 1, 1981·The Journal of Membrane Biology·H Gögelein, W Van Driessche
Jan 1, 1981·The Journal of Membrane Biology·H Gögelein, W Van Driessche
Oct 19, 1978·Nature·W Van Driessche, H Gögelein

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