Electrical Stimulation of Denervated Rat Skeletal Muscle Retards Capillary and Muscle Loss in Early Stages of Disuse Atrophy

BioMed Research International
Kouki NakagawaHideaki Onishi

Abstract

The purpose of the present study is to investigate the effects of low-frequency electrical muscle stimulation (ES) on the decrease in muscle mass, fiber size, capillary supply, and matrix metalloproteinase (MMP) immunoreactivity in the early stages of denervation-induced limb disuse. Direct ES was performed on the tibialis anterior muscle following denervation in seven-week-old male rats. The rats were divided into the following groups: control (CON), denervation (DN), and denervation with direct ES (DN + ES). Direct ES was performed at an intensity of 16 mA and a frequency of 10 Hz for 30 min per day, six days a week, for one week. We performed immunohistochemical staining to determine the expression of dystrophin, CD34, and MMP-2 in transverse sections of TA muscles. The weight, myofiber cross-sectional area (FCSA), and capillary-to-fiber (C/F) ratio of the tibialis anterior (TA) muscle were significantly reduced in the DN group compared to the control and DN + ES groups. The MMP-2 positive area was significantly greater in DN and DN + ES groups compared to the control group. These findings suggest beneficial effects of direct ES in reducing muscle atrophy and capillary regression without increasing MMP-2 immunoreactivity in ...Continue Reading

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Citations

Jan 10, 2018·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Keishi HayaoHideaki Onishi
Jul 22, 2018·Journal of Cachexia, Sarcopenia and Muscle·Volker Adams
Aug 2, 2019·BioResearch Open Access·Anjali PatelKoyal Garg
Mar 23, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Fan LiQingyan Jiang

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