Electrical Stimulation Promotes Cardiac Differentiation of Human Induced Pluripotent Stem Cells

Stem Cells International
Damián HernándezShiang Y Lim

Abstract

Background. Human induced pluripotent stem cells (iPSCs) are an attractive source of cardiomyocytes for cardiac repair and regeneration. In this study, we aim to determine whether acute electrical stimulation of human iPSCs can promote their differentiation to cardiomyocytes. Methods. Human iPSCs were differentiated to cardiac cells by forming embryoid bodies (EBs) for 5 days. EBs were then subjected to brief electrical stimulation and plated down for 14 days. Results. In iPS(Foreskin)-2 cell line, brief electrical stimulation at 65 mV/mm or 200 mV/mm for 5 min significantly increased the percentage of beating EBs present by day 14 after plating. Acute electrical stimulation also significantly increased the cardiac gene expression of ACTC1, TNNT2, MYH7, and MYL7. However, the cardiogenic effect of electrical stimulation was not reproducible in another iPS cell line, CERA007c6. Beating EBs from control and electrically stimulated groups expressed various cardiac-specific transcription factors and contractile muscle markers. Beating EBs were also shown to cycle calcium and were responsive to the chronotropic agents, isoproterenol and carbamylcholine, in a concentration-dependent manner. Conclusions. Our results demonstrate that b...Continue Reading

References

Jan 1, 1977·Annual Review of Biophysics and Bioengineering·L F Jaffe, R Nuccitelli
Nov 26, 1999·Journal of Cellular Biochemistry·H SauerM Wartenberg
Nov 4, 2006·Pharmacology & Therapeutics·Sian E HardingJulia Gorelik
Nov 22, 2007·Science·Junying YuJames A Thomson
Apr 4, 2008·Biochemical and Biophysical Research Communications·Jorge A GenoveseAmit N Patel
Feb 14, 2009·Circulation Research·Jianhua ZhangTimothy J Kamp
Sep 30, 2009·Circulation·Limor ZwiLior Gepstein
Feb 5, 2011·Nature Biotechnology·Gabriella L BoultingKevin Eggan
Apr 5, 2011·Nature Methods·Keisuke OkitaShinya Yamanaka
May 20, 2011·Nature·Michael A Laflamme, Charles E Murry
May 24, 2011·Stem Cells International·Kristiina RajalaKatriina Aalto-Setälä
Jan 30, 2014·Biotechnology and Bioengineering·A PavesiG B Fiore
Feb 1, 2014·Circulation Research·Xiulan YangCharles E Murry

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Citations

Sep 8, 2016·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Andrea BarbutiPatrizia Dell'Era
Oct 5, 2016·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ksenia BlinovaDavid G Strauss
Oct 17, 2018·Journal of Biomedical Materials Research. Part a·Pamela HitscherichEun Jung Lee
Feb 5, 2019·Journal of Cellular Physiology·Soraya NekouianSamad Nadri
Sep 15, 2017·Advanced Healthcare Materials·Armin GerailiAmir Sanati-Nezhad
Oct 3, 2019·American Journal of Physiology. Cell Physiology·Simon P WellsJames R Bell
May 18, 2020·Chemical Society Reviews·Lingyu SunYuanjin Zhao
May 22, 2020·Circulation Research·Rebecca H Ritchie, E Dale Abel
Aug 28, 2020·Frontiers in Bioengineering and Biotechnology·Pilar MonteroManuel M Mazo
Mar 21, 2019·Frontiers in Cell and Developmental Biology·Cristiana MatteiMirella Dottori
Apr 27, 2019·Journal of Tissue Engineering·Marc DwengerBradley B Keller
Apr 10, 2020·Current Stem Cell Research & Therapy·Weidong ZhangFengxuan Han
Feb 26, 2021·Pflügers Archiv : European journal of physiology·Kalina AndrysiakJózef Dulak
Apr 4, 2021·International Journal of Molecular Sciences·Emma Claire JamesJeremy Micah Crook
Apr 23, 2021·Acta Biomaterialia·Yimeng LiLu Wang
May 1, 2021·International Journal of Molecular Sciences·Kasturi MarkandranChristine Cheung

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Methods Mentioned

BETA
transfection
Polymerase Chain Reaction
SMA

Software Mentioned

Graphpad Prism
MC Rack

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