Electrically detected displacement assay (EDDA): a practical approach to nucleic acid testing in clinical or medical diagnosis

Analytical and Bioanalytical Chemistry
P LiepoldG Hartwich

Abstract

This paper introduces the electrically detected displacement assay (EDDA), a electrical biosensor detection principle for applications in medical and clinical diagnosis, and compares the method to currently available microarray technologies in this field. The sensor can be integrated into automated systems of routine diagnosis, but may also be used as a sensor that is directly applied to the polymerase chain reaction (PCR) reaction vessel to detect unlabeled target amplicons within a few minutes. Major aspects of sensor assembly like immobilization procedure, accessibility of the capture probes, and prevention from nonspecific target adsorption, that are a prerequisite for a robust and reliable performance of the sensor, are demonstrated. Additionally, exemplary results from a human papillomavirus assay are presented.

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Citations

Mar 25, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Nadir MeiniNicole Jaffrezic-Renault
Aug 24, 2010·Biosensors & Bioelectronics·Philipp KruppaDoris Schmitt-Landsiedel
Aug 26, 2015·ACS Applied Materials & Interfaces·Katarzyna BialaGerd-Uwe Flechsig
Oct 18, 2016·Chemistry : a European Journal·Qiang Su, Gilbert Nöll
Mar 28, 2013·Angewandte Chemie·Yaming YuGilbert Nöll
Jul 17, 2015·Journal of Clinical Imaging Science·Lih En HongJordan Li
Jul 23, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Donny Nugraha MazaafriantoManabu Tokeshi
Mar 18, 2011·Faraday Discussions·Magdalena GebalaWolfgang Schuhmann
Jun 17, 2011·Small·Miao-Ping Chien, Nathan C Gianneschi
Feb 18, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Ramya VishnubhotlaSteve Semancik
Jul 15, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Qiang SuGilbert Nöll
Nov 5, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Qiang SuGilbert Nöll

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