Electroacupuncture increases CB2 receptor expression on keratinocytes and infiltrating inflammatory cells in inflamed skin tissues of rats

The Journal of Pain : Official Journal of the American Pain Society
Jing ZhangM Li

Abstract

Endogenous cannabinoids and peripheral cannabinoid CB2 receptors (CB2Rs) are involved in the antinociceptive effect of electroacupuncture (EA) on inflammatory pain. However, it remains unclear about how EA affects the expression and distribution patterns of peripheral CB2Rs in inflamed skin tissues. To study this, inflammatory pain was induced by local injection of complete Freund's adjuvant into the hindpaw of rats. The mRNA and protein levels of CB2Rs were quantified by using RTPCR and Western blotting, respectively. The distribution of CB2Rs on keratinocytes and immune cells recruited to the inflamed skin tissues was determined by using double-immunofluorescence labeling. Induction of tissue inflammation significantly increased the mRNA and protein levels of CB2Rs in the skin tissue. Also, both 2 Hz and 100 Hz EA, applied to GB30 and GB34, significantly increased the mRNA and protein levels of CB2Rs in inflamed tissues compared to the sham EA group. CB2Rimmunoreactivities were mainly distributed in keratinocytes, macrophages, and T-lymphocytes in the epidermis and dermis of the inflamed skin tissue. Inflammation caused a significant increase in the number of CB2R-immunoreactive keratinocytes, macrophages, and T-lymphocytes. ...Continue Reading

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Citations

Aug 14, 2012·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Ping PengShiying Yu
Nov 15, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Branden A SmeesterAlvin J Beitz
Dec 26, 2012·Journal of Acupuncture and Meridian Studies·Lawrence Leung
Jun 9, 2016·Journal of Neuroinflammation·Ya-Qun ZhouYu-Ke Tian
Dec 17, 2008·Journal of Bodywork and Movement Therapies·John M McPartland
Dec 11, 2013·Anesthesiology·Ruixin ZhangBrian M Berman
May 26, 2018·Current Opinion in Anaesthesiology·Bihua BieMohamed Naguib
Mar 9, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kinga Fanni TóthAttila Oláh
Oct 19, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jia SunDa-Wei Ye
Jan 22, 2021·Pflügers Archiv : European journal of physiology·Maísa Maria Spagnol TrentoDaniel Fernandes Martins
Mar 9, 2021·Frontiers in Neuroscience·Iona J MacDonald, Yi-Hung Chen

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