Electroacupuncture Preconditioning Improves Myocardial Infarction Injury via Enhancing AMPK-Dependent Autophagy in Rats

BioMed Research International
Qing ZengGuo-Zhi Huang

Abstract

Electroacupuncture (EA) pretreatment plays a protective role in myocardial infarction injury. However, the mechanism of electroacupuncture remains unknown. The aim of this study was to confirm the protective effects of electroacupuncture (EA) on myocardial infarction injury and the possible mechanism. Sprague-Dawley (SD) rats, used to serve as acute myocardial infarction (AMI) model, were divided into sham group, model (M) group, M+EA group, AMPK inhibitor Compound C (M+EA+CC), and AMPK inhibitor solvent control (M+EA+DMSO) group, respectively. Rats in EA group were pretreated with EA and those in M+EA+CC group with intravenous AMPK inhibitor Compound C. The myocardial morphological changes and infarct size were observed through HE staining and TTC staining, and the concentrations of CK-MB and LDH were detected using ELISA kits. Transmission electron microscopy was employed to observe the autophagosome formation, and the AMPK-dependent autophagy-related protein expression was detected by immunohistochemistry and western blot. EA could alleviate myocardial infarction injury and decrease the concentrations of CK-MB and LDH. Transmission electron microscopy showed that EA could also regulate the AMPK-dependent autophagosome format...Continue Reading

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Citations

Jul 2, 2019·Molecular Medicine Reports·Lanfei ZhouYisen Shao
Dec 31, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Qiu-Fu DaiXiao-Chun Yu
Jul 14, 2020·Cardiology Research and Practice·Zhendong FengWei Jing Liu
Jan 8, 2021·Frontiers in Neuroscience·Chao-Chao YuLi-Hong Kong

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Methods Mentioned

BETA
ELISA
protein collection
protein assay
electrophoresis
transmission electron microscopy
transgenic

Software Mentioned

SPSS17
Pro Plus
Photoshop
Image

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