Electroacupuncture Reduces Weight in Diet-Induced Obese Rats via Hypothalamic Tsc1 Promoter Demethylation and Inhibition of the Activity of mTORC1 Signaling Pathway

Evidence-based Complementary and Alternative Medicine : ECAM
Jincheng LengYing Li

Abstract

The study aimed to investigate the mechanism of electroacupuncture reducing weight via tuberous sclerosis complex 1 (Tsc1) promoter methylation, inhibiting the mammalian target of rapamycin complex 1 (mTORC1) pathway. Male Sprague-Dawley rats were divided into chow-fed group (chow group) or high-fat diet group (HF group) for 14 weeks. The obesity rats in HF group were randomly divided into electroacupuncture group (EA group) and diet-induced obesity (DIO) group, which received EA stimulation on bilateral ST25, RN12, SP6, and ST36 for 4 weeks or no further treatment, respectively. Methylation of the Tsc1 gene promoter and expression of agouti-related protein (AgRP), neuropeptide Y (NPY), and proopiomelanocortin (PoMC) were detected at the 18th week. At week 18, weight, body fat, and the body fat rate in DIO group were significantly higher than those of the chow and EA group. Compared with the chow group, the DIO group had increased methylation of the Tsc1 gene promoter and expression of mTORC1, AgRP, and NPY gene and decreased PoMC in the hypothalamus; after EA, methylation of the Tsc1 gene promoter, mRNA, and protein of the mTORC1 and expression of AgRP and NPY gene decreased and PoMC increased significantly. Our study could sh...Continue Reading

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Citations

Jul 30, 2020·BioMed Research International·Lin-Lin XieXian-Lun Pang
Mar 12, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Li-Hua WangZhong-Yu Zhou
Oct 31, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Yin ShouBimeng Zhang
Apr 20, 2021·Frontiers in Endocrinology·Li WangYan-Jun Du
Jun 1, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Yuan-Chieh YehTse-Hung Huang

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Methods Mentioned

BETA
immunoprecipitation
PCR
Biopsy
GTPase

Software Mentioned

GraphPad Prism
Gel image imaging
Sequence Detection

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