Electroacupuncture regulates apoptosis/proliferation of intramuscular interstitial cells of cajal and restores colonic motility in diabetic constipation rats

Evidence-based Complementary and Alternative Medicine : ECAM
Juan Juan XuXiao Hua Hou

Abstract

Injury of interstitial cells of Cajal (ICC) is associated with gut dysmotility in diabetic rats. We have shown an acceleration of the colonic contractility by electroacupuncture stimulation (EAS). However, little is known about potential roles of EAS on colonic transit and ICC. In this study, we evaluate the effect of EAS on colonic transit and investigate whether apoptosis/proliferation of ICC was involved in regulative effect of EAS on colonic transit. Rats were randomly assigned to normal, diabetic, diabetic-plus-sham stimulation, diabetic-plus-low-frequency stimulation, and diabetic-plus-high-frequency stimulation groups. Bead expulsion test was used for measuring the distal colonic transit. The Kit (ICC marker) was detected by western blot. Apoptotic ICC was detected by terminal dUTP nucleotide end labeling. Proliferating ICC was identified by Kit/Ki67 double immunofluorescent staining on whole mount preparations. Ultrastructure changes of ICC were studied using electron microscopy. Results showed that high-frequency stimulation significantly promoted colonic transit. Low- and high-frequency stimulation markedly rescued intramuscular ICC from apoptosis. Abundant proliferating intramuscular ICC was found in low- and high-fr...Continue Reading

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Citations

Feb 17, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Lugao TianShi Liu
Jan 24, 2019·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Jing AnShi Liu
Jun 15, 2016·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Guoshan ZhangXiaorong Chang
Mar 29, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Xinjun WangJiande D Z Chen
May 15, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Fan Du, Shi Liu
Mar 10, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Shan LiuHuali Xu
Jan 24, 2021·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Shuangning SongShi Liu
May 16, 2021·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Yiling ZhangJiande D Z Chen

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Methods Mentioned

BETA
electron microscopy
electrophoresis
dissection

Software Mentioned

Image
Pro plus

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

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