Electroanalytical behavior of poly-L-lysine dendrigrafts at the interface between two immiscible electrolyte solutions

Analytical Chemistry
Grégoire HerzogDamien W M Arrigan

Abstract

In this work, the electrochemical behavior of nonredox-active poly-L-lysine dendrigraft molecules of four different generations was investigated at the interface between two immiscible electrolyte solutions (ITIES). The influence of the dendrigraft generation on the electrochemical response, sensitivity of the calibration curves, and limit of detection was studied. Cyclic voltammetry at the ITIES revealed that the sensitivity increased (1840 to 25 800 nA μM(-1)) and the limit of detection decreased (11.10 to 0.65 μM) as the dendrigraft generation increased from generation G2 through to generation G5, respectively. The results are compared to those for protein voltammetry at the ITIES. Our studies suggest that the sensitivity expected for a synthetic ionized macromolecule can be predicted on the basis of its net charge and its diffusion coefficient. However, electrochemistry at the ITIES demonstrates a greater sensitivity toward proteins, which is attributed to their tertiary structure.

References

Mar 19, 2004·Journal of Colloid and Interface Science·Sanna HakkarainenKyösti Kontturi
Mar 19, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Takuya KasaharaTakashi Kakiuchi
Jun 14, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Mariko ShinshiMasahiro Goto
May 23, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Alfonso BerduqueDamien W M Arrigan
May 22, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Marcelo CalderonLidia M Yudi
Aug 1, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Francine KivlehanDamien W M Arrigan
Aug 19, 2008·Journal of the American Chemical Society·Jeremy L EnglandGilad Haran
Oct 14, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Hirohisa NagataniTakamasa Sagara
Sep 3, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Limin ZhangTakashi Kakiuchi
Dec 22, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Rune A HartvigHenrik Jensen

Related Concepts

Electrolytes
Poly-(Alpha-L-Lysine)
Dendrimers
Electrochemical Techniques
Limit of Detection
Cyclic Peptides
Poly ICL
Tertiary Protein Structure
Macromolecule
Intermediate

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