Electrochemical analysis of hypothalamic serotonin metabolism accompanied by immobilization stress in rats.

Physiology & Behavior
N ShimizuK Aoyagi

Abstract

The effects of stress on serotonin metabolism in the lateral hypothalamic area (LHA) were investigated. Differential pulse voltammetry with a carbon fiber electrode was used to measure 5-hydroxyindoleacetic acid (5-HIAA), the metabolic product of the serotonin (5-HT). High-performance liquid chromatography with electrochemical detection was also used to analyze these compounds in the cerebrospinal fluid (CSF). The peak in voltammogram at an oxidation potential of +230 mV (P3) was identified as 5-HIAA by pharmacological manipulations that are known to affect 5-HT metabolism. A significant increase in 5-HIAA concentration in the LHA was detected after immobilization. An intraperitoneal injection of 5-hydroxytryptophan (5-HTP; a precursor of 5-HT) increased the height of P3, and injection of pargyline prevented the effect of 5-HTP during the course of increasing P3. These results support our previous conclusion that immobilization-induced anorexia might be mediated through activation of serotonergic mechanisms in the LHA.

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Citations

Jun 1, 1993·Biulleten' eksperimental'noĭ biologii i meditsiny·A S SosnovskiĭS S Pertsov
Dec 1, 1990·Pharmacology, Biochemistry, and Behavior·S F LeibowitzJ S Suh
Jan 1, 1996·Neuroscience and Biobehavioral Reviews·L L Bernardis, L L Bellinger
Mar 17, 2010·The Journal of Experimental Biology·Ian C HallLaura M Hurley
Mar 5, 2021·Behavioural Pharmacology·Ernesto Limón-BernalHéctor I Rocha-González

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