Electrochemical aptasensor of cardiac troponin I for the early diagnosis of acute myocardial infarction

Analytical Chemistry
Hunho JoChangill Ban

Abstract

Cardiac troponin I (cTnI) is well-known as a promising biomarker for the early diagnosis of acute myocardial infarction (AMI). In this work, single-stranded DNA aptamers against cTnI were identified by the Systematic Evolution of Ligands by Exponential enrichment (SELEX) method. The aptamer candidates exhibited a high selectivity and sensitivity toward both cTnI and the cardiac Troponin complex. The binding affinities of each aptamer were evaluated based on their dissociation constants (Kd) by surface plasma resonance. The Tro4 aptamer that had the highest binding capacity to cTnI showed a very low Kd value (270 pM) compared with that of a cTnI antibody (20.8 nM). Furthermore, we designed a new electrochemical aptasensor based on square wave voltammetry using ferrocene-modified silica nanoparticles. The developed aptasensor demonstrated an excellent analytical performance for cTnI with a wide linear range of 1-10 000 pM in a buffer and a detection limit of 1.0 pM (24 pg/mL; S/N = 3), which was noticeably lower than the cutoff values (70-400 pg/mL). The specificity of the aptamers was also examined using nontarget proteins, demonstrating that the proposed sensor responded to only cTnI. In addition, cTnI was successfully detected...Continue Reading

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Citations

Feb 28, 2016·Biosensors & Bioelectronics·Hunho JoChangill Ban
May 8, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yanxue CaiXiaowei He
May 7, 2016·Experimental & Molecular Medicine·Hunho Jo, Changill Ban
Apr 19, 2020·Photochemistry and Photobiology·Vasilisa V KrasitskayaLudmila A Frank
Jul 18, 2020·International Journal of Molecular Sciences·Zoltán János TolnaiTamás Mészáros
Feb 20, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zheng WeiLiran Xu
Nov 24, 2019·Scientific Reports·Muthaiyan LakshmanakumarJohn Bosco Balaguru Rayappan
Nov 17, 2016·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Jingping HuZhiguang Zhou
Jun 26, 2020·Frontiers in Bioengineering and Biotechnology·Chaohong ShiJingjing Zhang
Jan 15, 2021·Diagnostics·Ragaa Abd-Ellatief, Maha Ragaa Abd-Ellatief
Mar 24, 2021·ACS Biomaterials Science & Engineering·Ji Hye YumSoyoung Park
Mar 10, 2019·Biosensors & Bioelectronics·Sabine SzuneritsRabah Boukherroub
Nov 21, 2016·International Journal of Biological Macromolecules·Deepika BhatnagarAshok Kumar
Oct 16, 2018·ACS Omega·Iwona GrabowskaSabine Szunerits
Jan 21, 2017·Analytical Chemistry·Yan Du, Shaojun Dong

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