Electrochemical behaviour of hen-egg-white lysozyme at the polarised water/1,2-dichloroethane interface

Physical Chemistry Chemical Physics : PCCP
Micheál D ScanlonDamien W M Arrigan

Abstract

The electrochemical behaviour of hen-egg-white lysozyme (HEWL) was studied at the polarized water/1,2-dichloroethane interface. The voltammetric ion-transfer response was found to be dependent on the pH and ionic strength of the aqueous phase solution and also on the nature of the organic phase electrolyte anion. The current-pH behaviour of HEWL was dominated by the charge of the biomolecule at each pH, as indicated by the close relationship between the experimental peak currents and the theoretical curve for HEWL based on its known acid-base chemistry. Three organic electrolyte anions of differing hydrophobicities were investigated (TFPB-, TPBCl- and TPB-) and it was found that the ion transfer voltammetric peaks occurred at successively higher potentials in the order of increasing hydrophobicity, Deltaphi(TPB) < Deltaphi(TPBCl) < Deltaphi(TPBF). The voltammetric response was time dependent during multi-cyclic voltammetry experiments, with the formation of a white film of precipitate at the interface. A pre-peak consistent with adsorption of the HEWL ion transfer product at the liquid/liquid interface was also observed. The results suggest that an adsorption or re-arrangement of HEWL molecules with time at the interface is tak...Continue Reading

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Citations

Dec 15, 2012·Analytical and Bioanalytical Chemistry·Eva Alvarez de EulateDamien W M Arrigan
Dec 25, 2012·Analytical Chemistry·Shane O'Sullivan, Damien W M Arrigan
Mar 8, 2016·The Journal of Physical Chemistry. B·Mahreen AroojRicardo L Mancera
Sep 4, 2015·Lab on a Chip·Mayte Conejero-MurielJosé A Gavira
Jun 11, 2010·Physical Chemistry Chemical Physics : PCCP·Micheál D ScanlonDamien W M Arrigan
Oct 7, 2010·Physical Chemistry Chemical Physics : PCCP·Shaikh Nayeem FaisalHye Jin Lee
Jul 26, 2012·Chemistry, an Asian Journal·Eva Alvarez de EulateDamien W M Arrigan

Related Concepts

Ethylene dichloride, ion (1+)
Lysozyme, chicken egg white
Electrochemistry
Electrolytes
Ethylene Dichlorides
Hydrogen-Ion Concentration
Leftose
Organic Chemicals
Osmolality
Acids

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