Electrochemical detection of D-dimer as deep vein thrombosis marker using single-chain D-dimer antibody immobilized on functionalized polypyrrole

Biosensors & Bioelectronics
S ChebilHafsa Korri-Youssoufi

Abstract

We describe a rapid and sensitive method for detection and quantification of d-dimer which is a biomarker present at elevated concentrations in patients with deep vein thrombosis (DVT) disorders. The method uses an immunosensor based on a single-chain antibody (ScAb) immobilized on a transducer surface and with a densely packed receptor layer. Detection is based on the redox activity of a N-alpha bis(carboxymethyl)-L-lysine (ANTA)/Cu2+ complex attached to a polypyrrole backbone. The resulting hybrid material: polypyrrole ANTA/metal complex/His-tag ScAb was characterized by AFM, surface plasmon resonance (SPR) and differential pulse voltammetry (DPV) for the optimization of the biosensor formation. The biosensor offers a promising template for antibody immobilization and for immunodetection of a specific D-dimer. The biosensor shows a remarkable variation in redox activity of the ANTA/Cu2+ complex after the D-dimer association with a binding constant Kd of 1 ng mL(-1). Electrochemical impedance spectroscopy (EIS) allows monitoring D-dimer association with a linear response between 0.1 ng mL(-1) and 500 ng mL(-1) and a detection limit of 100 pg mL(-1) in PBS is obtained. The biolayer exhibits the same sensitivity for the detectio...Continue Reading

Citations

Jan 31, 2015·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Yang FeiRen-Min Liu
Jul 2, 2016·Essays in Biochemistry·Shikha SharmaRichard J O'Kennedy
Feb 15, 2014·The Analyst·Palaniappan SubramanianSabine Szunerits
Jan 12, 2011·Chemical Society Reviews·Serge Cosnier, Michael Holzinger
Feb 2, 2021·Trends in Analytical Chemistry : TRAC·Sharda KotruMatiar M R Howlader
Jun 8, 2021·Current Opinion in Colloid & Interface Science·Ebru Saatçi, Satheesh Natarajan
Sep 22, 2021·ACS Applied Materials & Interfaces·Molly KozminskyLydia L Sohn
Oct 8, 2021·Analytical and Bioanalytical Chemistry·Ajay Kumar YagatiMin-Ho Lee

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