Electrochemical Detection of NG -Hydroxy-L-arginine

ECS Transactions
Mariah L Arral, Jeffrey M Halpern

Abstract

NG-hydroxy-L-arginine (NOHA) is a stable intermediate product in the consumption of L-arginine in the urea cycle by nitric oxide synthase (NOS) to produce nitric oxide (NO) and L-citrulline. Research has shown that the urea cycle is disrupted in various diseases. As one of the few electrochemically active species in the urea cycle, NOHA shows promise as a marker for detection of various diseases. Electrochemical detection is an established, cost-effective method that is able to successfully detect low levels of analyte concentrations. NOHA, to the best of our knowledge, has not been electrochemically detected previously. Using cyclic voltammetry with a glassy carbon electrode, we have found that NOHA has an oxidation peak at 355 mV with a sensitivity of 5.4 nA/μM. We also investigated detecting NOHA with differential pulse voltammetry, which shows similar sensitivity and oxidation peaks. While there is significant work ahead to understand the kinetics of NOHA detection, the results here represent the first steps in making a NOHA biosensor.

Citations

Jun 4, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Israel Pérez-TorresVerónica Guarner-Lans
Feb 26, 2020·Journal of the Electrochemical Society·Mariah L ArralJeffrey Mark Halpern
Apr 9, 2020·Journal of the Electrochemical Society·Joelle M J LaFreniereJeffrey M Halpern
Jul 27, 2021·Advanced Healthcare Materials·Ning TangHossam Haick

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