Electrochemical detection of oligopeptides at silicon-fabricated micro-liquid/liquid interfaces

Analytical Chemistry
Micheál D ScanlonDamien W M Arrigan

Abstract

The detection of peptides is an important bioanalytical challenge, as they are a generic class of potent molecules of biomedical and biopharmaceutical significance. In this work, the electrochemistry of seven oligopeptides at microscaled interfaces between two immiscible electrolyte solutions (microITIES) was investigated. Their transfer across the polarized interface was assisted by dibenzo-18-crown-6 (DB18C6). The ion transfer potentials of these oligopeptides were dependent on their hydrophobicities and their interaction with DB18C6. Micropore arrays, which were fabricated in silicon by a combination of wet and dry etch techniques, were used to enhance mass transfer and thus analytical sensitivities. The use of a gellified organic phase allowed the implementation of voltammetric stripping techniques at the liquid-organogel interface. The combination of interface miniaturization and stripping voltammetry provided limits of detection at submicromolar concentration levels. The sensitivities (calibration graph slopes) were -3205 nA microM(-1) cm(-2) for Phe-Phe, -1791 nA microM(-1) cm(-2) for Leu-Leu, -6014 nA microM(-1) cm(-2) for Lys-Lys, and -9611 nA microM(-1) cm(-2) for Lys-Lys-Lys. Mixtures of peptides were also investigat...Continue Reading

References

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Citations

Dec 15, 2012·Analytical and Bioanalytical Chemistry·Eva Alvarez de EulateDamien W M Arrigan
Mar 19, 2013·Analytica Chimica Acta·Grégoire Herzog, Valerio Beni
Dec 25, 2012·Analytical Chemistry·Shane O'Sullivan, Damien W M Arrigan
Dec 24, 2013·Electrochimica Acta·Shigeru AmemiyaRyoichi Ishimatsu
Mar 24, 2009·Physical Chemistry Chemical Physics : PCCP·Micheál D ScanlonDamien W M Arrigan
Sep 4, 2009·Electrophoresis·Garry D SiskDamien W M Arrigan
Jun 11, 2010·Physical Chemistry Chemical Physics : PCCP·Micheál D ScanlonDamien W M Arrigan
Oct 7, 2010·Physical Chemistry Chemical Physics : PCCP·Shaikh Nayeem FaisalHye Jin Lee
Mar 11, 2011·Chemical Society Reviews·Shujuan LiuYuanhua Shao
Dec 18, 2020·Bioelectrochemistry·Shaheda Zannah, Damien W M Arrigan
Dec 17, 2009·Analytical Chemistry·Grégoire HerzogDamien W M Arrigan

Related Concepts

Polyether XXVIII
Electrochemistry
Oligopeptides
Silicon
Solutions
Cryptands
Hydrophobicity
Biological Factors
Leucine
Lysine

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