Electrochemical detection of Pseudomonas in wound exudate samples from patients with chronic wounds

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Hunter J SismaetEdgar D Goluch

Abstract

In clinical practice, point-of-care diagnostic testing has progressed rapidly in the last decade. For the field of wound care, there is a compelling need to develop rapid alternatives for bacterial identification in the clinical setting, where it generally takes over 24 hours to receive a positive identification. Even new molecular and biochemical identification methods require an initial incubation period of several hours to obtain a sufficient number of cells prior to performing the analysis. Here we report the use of an inexpensive, disposable electrochemical sensor to detect pyocyanin, a unique, redox-active quorum sensing molecule released by Pseudomonas aeruginosa, in wound fluid from patients with chronic wounds enrolled in the WE-HEAL Study. By measuring the metabolite excreted by the cells, this electrochemical detection strategy eliminates sample preparation, takes less than a minute to complete, and requires only 7.5 μL of sample to complete the analysis. The electrochemical results were compared against 16S rRNA profiling using 454 pyrosequencing. Blind identification yielded 9 correct matches, 2 false negatives, and 3 false positives giving a sensitivity of 71% and specificity of 57% for detection of Pseudomonas. O...Continue Reading

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Citations

Mar 1, 2018·Annual Review of Analytical Chemistry·Hunter J Sismaet, Edgar D Goluch
Jan 18, 2019·Clinical Microbiology Reviews·Pranali J BuchEdgar D Goluch
May 11, 2020·Biotechnology Journal·Francis McEachernGeraldine Merle
May 15, 2018·Frontiers in Bioengineering and Biotechnology·Matthew S BrownAhyeon Koh
Oct 14, 2020·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Pranali J BuchEdgar D Goluch
Dec 1, 2020·Journal of Wound Care·Francisco R ÁvilaAntonio J Forte
Nov 19, 2020·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Vishaldeep KaurTapasi Sen
Nov 12, 2020·ACS Sensors·Olja SimoskaKeith J Stevenson
May 10, 2019·Analytical Chemistry·Martin K KimaniEdgar D Goluch
Nov 20, 2021·Science Advances·Ze XiongJohn S Ho

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