Electrochemical determination of S-nitrosothiols with a Clark-type nitric oxide electrode

Analytical Biochemistry
S PfeifferB Mayer

Abstract

Low-molecular-mass thiols and nitric oxide (NO) form S-nitrosothiols (thionitrites) in the presence of oxygen. Thionitrites play an integral role in a variety of NO-dependent physiological processes. This study describes a sensitive analytical method for the quantitative determination of thionitrites. The method is based on the Cu+-catalyzed homolytic cleavage of thionitrites and electrochemical detection of the released NO with a Clark-type electrode. Cu+ was generated by addition of Cu(NO3)2 to samples containing 1 mM GSH or 4 mM L-cysteine as reducing agents. The effect of Cu(NO3)2 on the release of NO from GSNO was concentration-dependent. In the presence of 1 mM GSH, the EC50 for Cu(NO3)2 was 1.34 +/- 0.08 mM. Using cysteine instead of GSH, NO release was quantitative at much lower concentrations of Cu(NO3)2 (EC50 = 8.5 +/- 2.8 microM. NO release was not significantly affected by pH (7.0-9.0) and was inhibited by the Cu+-selective chelator neocuproine, whereas the Cu2+ chelator cuprizone was approximately 16-fold less potent. Calibration of the method with GSNO, S-nitroso-N-acetyl-penicillamine, or S-nitrosated bovine serum albumin yielded linear plots of initial rates of NO release versus thionitrite concentration from 50...Continue Reading

References

Nov 1, 1992·British Journal of Pharmacology·M W RadomskiS Moncada
Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J S StamlerJ Loscalzo
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J S StamlerJ Loscalzo
Nov 24, 1995·The Journal of Biological Chemistry·V G KharitonovV S Sharma
Jan 13, 1995·The Journal of Biological Chemistry·B MayerK Schmidt
Sep 8, 1995·The Journal of Biological Chemistry·H M LanderA Novogrodsky
Mar 1, 1995·British Journal of Pharmacology·M P GordgeA Noronha-Dutra
Feb 20, 1995·Archives of Biochemistry and Biophysics·N Spear, S D Aust
Oct 1, 1994·The Journal of Clinical Investigation·J S ScharfsteinJ Loscalzo
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B GastonD J Singel
Jul 12, 1993·FEBS Letters·P C FordD M Stanbury
Mar 18, 1996·FEBS Letters·N HoggB Kalyanaraman
Mar 21, 1996·Nature·M F Perutz
Jul 1, 1996·Analytical Biochemistry·J A CookD A Wink
Jun 15, 1996·Archives of Biochemistry and Biophysics·A C GorrenB Mayer
Aug 2, 1996·The Journal of Biological Chemistry·R J SinghB Kalyanaraman
Mar 1, 1996·The American Journal of Physiology·C A DavidsonM S Wolin
Jun 1, 1996·British Journal of Pharmacology·A García-PascualD Triguero
Aug 1, 1996·Chemistry & Biology·A P Dicks, D L Williams
Sep 1, 1996·Chemical Research in Toxicology·M KeshiveW M Deen
Oct 1, 1996·British Journal of Pharmacology·M P GordgeA A Dutra
Nov 1, 1996·Biochemical and Biophysical Research Communications·Y HouP G Wang
Jun 15, 1997·Analytical Biochemistry·A R Butler, P Rhodes

❮ Previous
Next ❯

Citations

Dec 29, 2011·Analytical Chemistry·Daniel A RiccioMark H Schoenfisch
Jul 16, 2003·Clinical and Experimental Pharmacology & Physiology·Chi Wai NgChun Guang Li
Sep 11, 2003·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·J C Millar
Sep 23, 2014·Free Radical Biology & Medicine·Bernd KolesnikAntonius C F Gorren
Jan 11, 2003·Biotechnology & Genetic Engineering Reviews·Don J Durzan, M Cristina Pedroso
Jun 21, 2005·Nitric Oxide : Biology and Chemistry·Xiaoping LiuJay L Zweier
Apr 28, 2004·Free Radical Biology & Medicine·Ding-I YangChung Y Hsu
May 29, 2007·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Ke-Jing HuangHong Wang
Dec 25, 2002·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Hülya BayirValerian E Kagan
Feb 1, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Robson Cavalcante VerasIsac Almeida Medeiros
Sep 25, 1999·The Journal of Biological Chemistry·G StubauerP Sarti
Sep 14, 1999·The American Journal of Physiology·J YouR M Bryan
Nov 26, 2002·The Journal of Biological Chemistry·Jivko V Stoyanov, Nigel L Brown
Dec 22, 2004·The Journal of Biological Chemistry·Inga SliskovicBulent Mutus
Mar 4, 2000·Free Radical Biology & Medicine·D Jourd'heuilM B Grisham
Aug 6, 2000·Free Radical Biology & Medicine·N Hogg
Feb 26, 2015·Analytical Chemistry·Rebecca A Hunter, Mark H Schoenfisch

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.