Electrochemical extraction of proteins by reverse micelle formation

Langmuir : the ACS Journal of Surfaces and Colloids
Mariko ShinshiMasahiro Goto

Abstract

The transfer of proteins by the anionic surfactant bis(2-ethylhexyl) sulfosuccinate (AOT) at a polarized 1,2-dichloroethane/water (DCE/W) interface was investigated by means of ion-transfer voltammetry. When the tetrapentylammonium salt of AOT was added to the DCE phase, the facilitated transfer of certain proteins, including cytochrome c (Cyt c), ribonuclease A, and protamine, could be controlled electrochemically, and a well-defined anodic wave for the transfer was obtained. At low pH values (e.g., pH 3.4), the anodic wave was usually well-separated from the wave for the formation of protein-free (i.e., unfilled) reverse micelles. The anodic wave for the protein transfer was analyzed by applying the theory for facilitated transfer of ions by charged ligands and then supplying information regarding the number of AOT anions reacting with one protein molecule and the total charge carried by the protein transfer. However, controlled-potential electrolyses performed for the transfer of Cyt c, which is red, revealed that the protein-AOT complexes were unstable in DCE and liable to aggregate at the interface when the pH of the W phase was 3.4. At pH 7.0, when formation of unfilled reverse micelles occurred simultaneously, the protei...Continue Reading

Citations

Mar 19, 2013·Analytica Chimica Acta·Grégoire Herzog, Valerio Beni
Dec 25, 2012·Analytical Chemistry·Shane O'Sullivan, Damien W M Arrigan
Oct 4, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Manuel A MéndezHubert H Girault
Jun 27, 2007·The Analyst·Grégoire Herzog, Damien W M Arrigan
Mar 24, 2009·Physical Chemistry Chemical Physics : PCCP·Micheál D ScanlonDamien W M Arrigan
Dec 15, 2011·The Journal of Physical Chemistry. B·Yoko ImaiToshiyuki Osakai
Jul 26, 2012·Chemistry, an Asian Journal·Eva Alvarez de EulateDamien W M Arrigan
Jul 11, 2008·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Toshiyuki Osakai, Aya Shinohara
Dec 18, 2020·Bioelectrochemistry·Shaheda Zannah, Damien W M Arrigan

Related Concepts

Ethylene dichloride, ion (1+)
1,4-bis(2-ethylhexyl) sodium sulfosuccinate
Electrochemistry
Electrolysis
Ethylene Dichlorides
Hydrogen-Ion Concentration
Micelles
Protamine Sulfate (USP)
Pancreatic ribonuclease
Succinic Acids

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