Electrochemical high-content screening of nitric oxide release from endothelial cells

Chembiochem : a European Journal of Chemical Biology
Sabine BorgmannWolfgang Schuhmann

Abstract

Release of nitric oxide (NO) is of high importance for regulating endothelial cell functions during vasodilatation, vascular remodeling, and angiogenesis. Thus, a direct and reliable real-time method for NO detection that takes into account time-dependent variations of the NO concentration in the complex reaction within the diffusion zone above the cells is vital for obtaining information about the role of NO in intracellular endothelial signal transduction and its impact on the surrounding cells. In this study, the time course of vascular endothelial growth factor E (VEGF-E) stimulated NO release from transformed human umbilical vein endothelial cells (T-HUVEC) was investigated by means of metalloporphyrin-based NO sensors employed in an electrochemical robotic system. The NO sensor was obtained by electrochemically induced deposition of Ni(II) tetrakis(p-nitrophenylporphyrin) on a 50-microm diameter platinum disk electrode which was integrated, together with a 25-microm diameter platinum disk, in a double-barrel electrode arrangement. The second electrode was used as a guidance sensor for the automatic and highly reproducible positioning of the NO sensor at a known distance from a layer of adherently growing cells by using z-...Continue Reading

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Citations

Mar 13, 2009·Analytical and Bioanalytical Chemistry·Sabine Borgmann
Feb 16, 2013·Analytical and Bioanalytical Chemistry·Frank MeinersGunther Wittstock
Jan 19, 2011·Analytica Chimica Acta·Sireerat IntarakamhangAlbert Schulte
Dec 1, 2006·Physiological Measurement·Martin A EdwardsPatrick R Unwin
Dec 8, 2017·Analytical and Bioanalytical Chemistry·Javier IzquierdoChristine Kranz
Jul 16, 2008·Chemical Reviews·Christian AmatoreFrédéric Lemaître

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