Electrochemical immunoassay for carcinoembryonic antigen based on signal amplification strategy of nanotubular mesoporous PdCu alloy

Biosensors & Bioelectronics
Yanyan CaiBin Du

Abstract

Interests in using nanoporous metals for biosensing applications have been increasing. Herein, nanotubular mesoporous PdCu (NM-PdCu) alloy is used to fabricate a novel label-free electrochemical immunosensor for cancer biomarker carcinoembryonic antigen (CEA). It operates through physisorption of anti-CEA on NM-PdCu and the mixture of sulfonated graphene sheets (HSO(3)-GS) and thionine (TH) functionalized glassy carbon electrode interface as the detection platform. In this study, chitosan (CS)-PdCu is bound very strongly to carcinoembryonic antibody (anti-CEA), because of the good electron conductivity, high surface area, and good biocompatibility. CS-PdCu is immobilized on electrodes by electrostatic interactions between the negatively charged sulfo group of HSO(3)-GS and the abundant positively charged amino groups of chitosan. TH acts as the redox probe. Under the optimized conditions, the electrochemical immunosensor exhibits a wide working range from 0.01 to 12 ng/mL with a low detection limit of 4.86 pg/mL. The accuracy, reproducibility, and stability of the immunosensor are acceptable. The assay is evaluated for real serum samples, receiving satisfactory results. The nanoporous metal materials-based immunoassay provides ...Continue Reading

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Citations

Dec 19, 2012·Analytica Chimica Acta·Xiaomei PeiDianping Tang
Dec 24, 2014·Bioanalysis·Ilaria Palchetti
Apr 8, 2017·Sensors·Benoît Piro, Steeve Reisberg
Jun 13, 2017·Natural Product Research·Wen-Ting LiYuan-Yuan Lu
Oct 14, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Hongmin MaQin Wei
Jan 14, 2021·Biomedical Physics & Engineering Express·Saloni TandonBalasubramanian Kandasubramanian

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