Electrochemical Immunoassay for Determination of Glycated Albumin using Nanozymes.

Scientific Reports
Hyun ChoiGi Hun Seong

Abstract

We developed a new nanozyme-based electrochemical immunoassay method for the monitoring of glycated albumin (GA) known to reflect short-term glycaemic levels. For this study, we synthesized urchin-like Pt nanozymes (uPtNZs) and applied them to colorimetric and electrochemical assays for sensitive determination of GA in total human serum albumin (tHSA) using 3,3',5,5'-tetramethylbenzidine (TMB) and thionine as substrates, respectively. The uPtNZs showed peroxidase-mimic activity in the presence of hydrogen peroxide. Boronic acid (BA)-agarose bead was used to capture GA through specific cis-diol interactions. uPtNZs were modified with GA antibody (GA-Ab) to form sandwich complexes with GA/BA-agarose bead. The amount of Ab-uPtNZ/GA/BA-agarose bead complex increased with increasing percentage of GA in 50 mg/mL tHSA. The colorimetric assay exhibited linearity from 0.02 to 10% (10 µg/mL - 5 mg/mL) GA with an LOD of 0.02% (9.2 µg/mL). For electrochemical assay, GA was detected from 0.01 to 20% (5 µg/mL - 10 mg/mL) with an LOD of 0.008% (3.8 µg/mL). The recovery values of measured GA in human plasma samples were from 106 to 107%. These results indicate that electrochemical assay using uPtNZs is a promising method for determining GA.

References

Dec 15, 1986·Clinica Chimica Acta; International Journal of Clinical Chemistry·P ReedP H Winocour
Nov 1, 1985·Annals of Clinical Biochemistry·P G Hill
Nov 5, 1982·Science·R Breslow
May 5, 1981·Clinica Chimica Acta; International Journal of Clinical Chemistry·R Dolhofer, O H Wieland
Sep 3, 2002·Clinica Chimica Acta; International Journal of Clinical Chemistry·Takuji KouzumaShigeyuki Imamura
Jun 24, 2004·Diabetic Medicine : a Journal of the British Diabetic Association·S L Jeffcoate
Jun 27, 2012·Chemistry : a European Journal·Huimin DengZhiqiang Gao
May 4, 2013·ACS Nano·Zhen GuDaniel G Anderson
Dec 4, 2014·Analytica Chimica Acta·Jong-Min ParkJae-Chul Pyun
Apr 7, 2016·Artificial Cells, Nanomedicine, and Biotechnology·Saeid Shabestari KhiabaniSoodabeh Davaran
Apr 23, 2017·Artificial Cells, Nanomedicine, and Biotechnology·Jafar GolchinAbolfazl Akbarzadeh
Jul 13, 2017·Archives of Endocrinology and Metabolism·Priscila Aparecida Correa FreitasJoíza Lins Camargo
Sep 26, 2017·ACS Applied Materials & Interfaces·Min Su KimJinwoo Lee
Jan 20, 2019·Journal of Colloid and Interface Science·Mithun SikderMohammed Baalousha

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