Electrochemical immunosensor based on gold-labeled monoclonal anti-LipL32 for leptospirosis diagnosis

Biosensors & Bioelectronics
Sakda JampasaNadnudda Rodthongkum

Abstract

Leptospirosis is a critical human health problem in the tropical area, thus, a precise technique that can be used for point-of-care analysis is greatly required. This is the first report on electrochemical immunosensor based on gold-labeled monoclonal anti-LipL32 for rapid, simple and sensitive determination of LipL32. The sensor consisted of two LipL32-specific antibodies: an unlabeled capture primary antibody (Anti-1°Ab) and an electrochemically detectable gold-conjugated secondary antibody (Au-2°Ab). The Anti-1°Ab was immobilized onto the modified screen-printed graphene electrode (SPGE) to form the anti-LipL32 surface. The electrochemical signal response was determined by differential pulse voltammetry (DPV). In the presence of LipL32, the sensor displayed a significant increase in current response in a concentration-dependent manner, but no observable signal was detected in the absence of LipL32. The linearity between LipL32 concentration and the measured current was found in a range of 1-100 ng/mL, and the limit of detection (LOD) (3SDblank/Slope) and limit of quantitation (LOQ) (10SDblank/Slope) were found to be 0.28 and 0.93 ng/mL, respectively. This sensor was successfully applied to detect pathogenic Leptospira whole ...Continue Reading

Citations

Jul 2, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Siraprapa BoobphahomKwanwoo Shin
Dec 12, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tamanna IslamA J Saleh Ahammad

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