Electrochemical immunosensor with aptamer-based enzymatic amplification

Analytical Biochemistry
Ke-Jun FengRu-Qin Yu

Abstract

An electrochemical immunosensor is reported by using aptamer-based enzymatic amplification with immunoglobulin E (IgE) as the model analyte. In this method, the IgE antibody is covalently immobilized as the capture probe on the gold electrode via a self-assembled monolayer of cysteamine. After the target is captured, the biotinylated anti-IgE aptamer is used as the detection probe. The specific interaction of streptavidin-conjugated alkaline phosphatase to the surface-bound biotinylated detection probe mediates a catalytic reaction of ascorbic acid 2-phosphate substrate to produce a reducing agent ascorbic acid. Then silver ions in the solution can be reduced, leading to the deposition of metallic silver on the electrode surface. The amount of deposited silver, which is determined by the amount of IgE target bound on the electrode surface, can be quantified using the stripping voltammetry. The results obtained demonstrated that the electrochemical immunosensor possesses high specificity and a wide dynamic range with a low detection limit that possibly arises from the combination of the highly specific aptamer and the highly sensitive stripping determination of enzymatically deposited silver.

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Citations

Dec 14, 2011·Analytical and Bioanalytical Chemistry·Xia WeiDanke Xu
Aug 25, 2009·Bioprocess and Biosystems Engineering·Yoon-Jin KimMan Bock Gu
Mar 24, 2009·Chemical Reviews·Juewen LiuYi Lu
Nov 19, 2010·Bioanalysis·Sonia CentiMarco Mascini
Dec 9, 2015·Nucleic Acid Therapeutics·Mariya VorobyevaAlya Venyaminova
Aug 14, 2012·Biosensors & Bioelectronics·Cheng-Yu LeeYou-Zung Hsieh
May 3, 2011·Analytical Biochemistry·Kyung-Mi SongChangill Ban
Jan 26, 2011·Chemical Society Reviews·Debasis Samanta, Amitabha Sarkar
Jan 7, 2019·Analytical and Bioanalytical Chemistry·Sathya SrinivasanBhaskar Mohan Murari
Aug 17, 2020·International Journal of Molecular Sciences·Zsófia Bognár, Róbert E Gyurcsányi
Aug 7, 2018·Analytical Biochemistry·Sathya SrinivasanBhaskar Mohan Murari
Jun 16, 2009·Journal of the American Chemical Society·Peter A SuciTrevor Douglas

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