PMID: 2096918Jul 1, 1990Paper

Electrochemical investigations of the interaction of metal chelates with DNA. 3. Electrogenerated chemiluminescent investigation of the interaction of tris(1,10-phenanthroline)ruthenium(II) with DNA

Bioconjugate Chemistry
M T Carter, A J Bard

Abstract

The electrogenerated chemiluminescence (ECL) that results from the oxidation of tris(1,10-phenanthroline)ruthenium(II), at a gold electrode in the presence of oxalate, was used to investigate the interaction of the Ru(II) chelate with calf thymus DNA. The decrease in ECL emission from the excited state, Ru(phen)3(2+*), in the presence of DNA, is ascribed to binding to binding of the chelate to the DNA strand. An ECL titration of the metal complex with DNA allowed determination of the equilibrium constant (K) and binding-site size (s) for association of Ru(phen)3(2+), under the assumption that only the free metal complex contributes to the observed emission. In 25 mM Na2C2O4, 2 mM phosphate buffer, pH 5, 0.05% Tween-20, 0.05% Triton X-100, regression based on the McGhee/von Hippel model, which accounts for free base pair gaps between binding sites, yielded K = 8.1 (+/- 0.2) x 10(3) M-1 and s = 4 bp.

References

Jan 1, 1985·Annual Review of Biophysics and Biophysical Chemistry·O G Berg, P H von Hippel
Apr 2, 1982·Molecular and Cellular Biochemistry·B C Baguley
Dec 1, 1983·Journal of Biomolecular Structure & Dynamics·J K Barton
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J K BartonA Alexandrescu

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Citations

Jan 15, 2011·Chemical Communications : Chem Comm·Cuisong ZhouXiaohong Fang
Aug 15, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shuyu ZhangHui Wei
Aug 16, 2016·Analytical and Bioanalytical Chemistry·Aila Jimenez-RuizConcepcion Torres-Marquez
Aug 10, 2004·Chembiochem : a European Journal of Chemical Biology·Chad E ImmoosMark W Grinstaff
Dec 9, 2016·Dalton Transactions : an International Journal of Inorganic Chemistry·Shu-Hua ZhangYu Xiao
Jul 2, 2010·Chemical Society Reviews·Lianzhe Hu, Guobao Xu
Sep 13, 2020·Biosensors·Emiliano Martínez-PeriñánEncarnación Lorenzo
Oct 8, 2019·Analytical Chemistry·Xian ChenHuanghao Yang
Oct 12, 2000·Analytical Chemistry·D W Pang, H D Abruña
Jan 1, 1997·Bioconjugate Chemistry·S O KelleyM G Hill
Sep 2, 2004·Journal of the American Chemical Society·Chad E ImmoosMark W Grinstaff
Dec 1, 2005·Analytical Chemistry·Jifeng LiuHubert H Girault

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